Tularemia in Denmark : identification of a Francisella tularensis subsp. holarctica strain by real-time PCR and high-resolution typing by multiple-locus variable-number tandem repeat analysis.

• We report ulceroglandular tularemia affecting an 8-year-old boy and the first recovery of Francisella tularensis in Denmark. A novel real-time PCR assay was used to identify the strain as F. tularensis subsp. holarctica (type B). Multiple-locus variable-number tandem repeat analysis demonstrated a close genetic relationship to strains from Norway.

Keyword

Bacterial Typing Techniques

Base Sequence

Child

Cluster Analysis

DNA; Bacterial/*analysis

Denmark

Francisella tularensis/*classification/genetics/isolation & purification

Humans

Male

Minisatellite Repeats/*genetics

Molecular Sequence Data

Polymerase Chain Reaction/*methods

Tularemia/*diagnosis/microbiology

Publication and Content Type

ref (subject category)

art (subject category)