T A microarray analysis of the murine macrophage response to infection with Francisella tularensis LVS

↓ Direkt till sidans innehåll
↓ Direkt till sidans sekundära innehåll (sidomenyn)

Search: onr:"swepub:oai:DiVA.org:umu-7833" > T A microarray anal...

1 of 1
Previous record
Next record
To hitlist

Details
MARC

Andersson, HenrikUmeå universitet,Klinisk bakteriologi (author)

T A microarray analysis of the murine macrophage response to infection with Francisella tularensis LVS

Article/chapterEnglish2006

Publisher, publication year, extent ...

Microbiology Society,2006
printrdacarrier

Numbers

LIBRIS-ID:oai:DiVA.org:umu-7833
https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-7833URI
https://doi.org/10.1099/jmm.0.46553-0DOI

Supplementary language notes

Language:English
Summary in:English

Part of subdatabase

SwePubSwePub

Classification

Subject category:ref swepub-contenttype
Subject category:art swepub-publicationtype

Notes

The response of cells of the mouse macrophage cell line J774 to infection with Francisella tularensis LVS was analysed by means of a DNA microarray representing approximately 18 500 genes (20 600 clones). The adaptive response was modest at all time points, and at most, 81 clones were differentially regulated from the time point of uptake of bacteria (0 min) up to 240 min later. For all five time points, 229 clones fulfilled the criteria of being differentially regulated, i.e. the ratio between infected versus non-infected cells was at least 1.7-fold up- or down-regulated and P <0.05. It was found that many of the differentially regulated genes are known to respond to stress in general and to oxidative stress specifically. However, at 120 min it was observed that genes that lead to depletion of glutathione were upregulated. Possibly, this was a result of mechanisms induced by F. tularensis. Generally, there was a conspicuous lack of inflammatory responses and, for example, although tumour necrosis factor alpha (TNF-α) was upregulated at 0 min, a significant down-regulation was noted at all subsequent time points. When cells were treated with an inhibitor of inducible nitric oxide synthase (iNOS) or the antioxidant N-acetylcysteine (NAC), the infection-induced cytopathogenic effect was significantly inhibited. Together, the results suggest that F. tularensis LVS infection confers an oxidative stress upon the target cells and that many of the host-defence mechanisms appear to be intended to counteract this stress. The infection is characterized by a very modest inflammatory response.

Added entries (persons, corporate bodies, meetings, titles ...)

Hartmanova (Andersson), BlankaUmeå universitet,Klinisk bakteriologi (author)
Landfors, MattiasUmeå universitet,Klinisk bakteriologi (author)
Ryden, PatrikUmeå universitet,Institutionen för matematik och matematisk statistik(Swepub:umu)pary0001 (author)
Noppa, LailaFOI, Umeå (Swedish Defence Research Agency) (author)
Näslund, LindaUmeå universitet,Klinisk bakteriologi (author)
Näslund, A. (author)
Umeå universitetKlinisk bakteriologi (creator_code:org_t)

Related titles

In:Journal of Medical Microbiology: Microbiology Society55:8, s. 1023-10330022-26151473-5644

Internet link

Find in a library

Journal of Medical Microbiology (Search for host publication in LIBRIS)

To the university's database

1 of 1
Previous record
Next record
To hitlist

Find more in SwePub

By the author/editor: Andersson, Henri ...; Hartmanova (Ande ...; Landfors, Mattia ...; Ryden, Patrik; Noppa, Laila; Näslund, Linda; show more...; Näslund, A.; show less...

Articles in the publication: Journal of Medic ...

By the university: Umeå University

Search outside SwePub

Extend your search to:: Google; Google Book Search; Google Scholar

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

LIBRIS.kb.se