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  • Hebels, Dennie G. A. J. (author)

Performance in omics analyses of blood samples in long-term storage : opportunities for the exploitation of existing biobanks in environmental health research

  • Article/chapterEnglish2013

Publisher, publication year, extent ...

  • Environmental Health Perspectives,2013
  • printrdacarrier

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  • LIBRIS-ID:oai:DiVA.org:umu-80868
  • https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-80868URI
  • https://doi.org/10.1289/ehp.1205657DOI

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  • Language:English
  • Summary in:English

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  • Subject category:ref swepub-contenttype
  • Subject category:art swepub-publicationtype

Notes

  • Background: The suitability for omic analysis of biosamples collected in previous decades and currently stored in biobanks is unknown.Objectives: We evaluated the influence of handling and storage conditions of blood-derived biosamples on transcriptomic, epigenomic (CpG methylation), plasma metabolomic [UPLC-ToFMS (ultra performance liquid chromatography-time-of-flight mass spectrometry)], and wide-target proteomic profiles.Methods: We collected fresh blood samples without RNA preservative in heparin, EDTA, or citrate and held them at room temperature for ≤ 24 hr before fractionating them into buffy coat, erythrocytes, and plasma and freezing the fractions at -80oC or in liquid nitrogen. We developed methodology for isolating RNA from the buffy coats and conducted omic analyses. Finally, we analyzed analogous samples from the EPIC-Italy and Northern Sweden Health and Disease Study biobanks.Results: Microarray-quality RNA could be isolated from buffy coats (including most biobank samples) that had been frozen within 8 hr of blood collection by thawing the samples in RNA preservative. Different anticoagulants influenced the metabolomic, proteomic, and to a lesser extent transcriptomic profiles. Transcriptomic profiles were most affected by the delay (as little as 2 hr) before blood fractionation, whereas storage temperature had minimal impact. Effects on metabolomic and proteomic profiles were noted in samples processed ≥ 8 hr after collection, but no effects were due to storage temperature. None of the variables examined significantly influenced the epigenomic profiles. No systematic influence of time-in-storage was observed in samples stored over a period of 13-17 years.Conclusions: Most samples currently stored in biobanks are amenable to meaningful omics analysis, provided that they satisfy collection and storage criteria defined in this study.

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Added entries (persons, corporate bodies, meetings, titles ...)

  • Georgiadis, Panagiotis (author)
  • Keun, Hector C. (author)
  • Athersuch, Toby J. (author)
  • Vineis, Paolo (author)
  • Vermeulen, Roel (author)
  • Portengen, Lützen (author)
  • Bergdahl, Ingvar A.Umeå universitet,Enheten för biobanksforskning,Yrkes- och miljömedicin,Arcum(Swepub:umu)inbe0001 (author)
  • Hallmans, GöranUmeå universitet,Enheten för biobanksforskning,Näringsforskning(Swepub:umu)goha0001 (author)
  • Palli, Domenico (author)
  • Bendinelli, Benedetta (author)
  • Krogh, Vittorio (author)
  • Tumino, Rosario (author)
  • Sacerdote, Carlotta (author)
  • Panico, Salvatore (author)
  • Kleinjans, Jos C. S. (author)
  • de Kok, Theo M. C. M. (author)
  • Smith, Martyn T. (author)
  • Kyrtopoulos, Soterios A. (author)
  • Umeå universitetEnheten för biobanksforskning (creator_code:org_t)

Related titles

  • In:Journal of Environmental Health Perspectives: Environmental Health Perspectives121:4, s. 480-4870091-67651552-9924

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