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Proteomic Amino-Ter...
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Huesgen, Pitter F.
(author)
Proteomic Amino-Termini Profiling Reveals Targeting Information for Protein Import into Complex Plastids
- Article/chapterEnglish2013
Publisher, publication year, extent ...
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2013-09-16
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Public Library of Science (PLoS),2013
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electronicrdacarrier
Numbers
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LIBRIS-ID:oai:DiVA.org:umu-82292
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https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-82292URI
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https://doi.org/10.1371/journal.pone.0074483DOI
Supplementary language notes
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Language:English
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Summary in:English
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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In organisms with complex plastids acquired by secondary endosymbiosis from a photosynthetic eukaryote, the majority of plastid proteins are nuclear-encoded, translated on cytoplasmic ribosomes, and guided across four membranes by a bipartite targeting sequence. In-depth understanding of this vital import process has been impeded by a lack of information about the transit peptide part of this sequence, which mediates transport across the inner three membranes. We determined the mature N-termini of hundreds of proteins from the model diatom Thalassiosira pseudonana, revealing extensive N-terminal modification by acetylation and proteolytic processing in both cytosol and plastid. We identified 63 mature N-termini of nucleus-encoded plastid proteins, deduced their complete transit peptide sequences, determined a consensus motif for their cleavage by the stromal processing peptidase, and found evidence for subsequent processing by a plastid methionine aminopeptidase. The cleavage motif differs from that of higher plants, but is shared with other eukaryotes with complex plastids.
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Added entries (persons, corporate bodies, meetings, titles ...)
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Alami, Meriem
(author)
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Lange, Philipp F.
(author)
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Foster, Leonard J.
(author)
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Schröder, Wolfgang P.Umeå universitet,Kemiska institutionen(Swepub:umu)wosc0001
(author)
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Overall, Christopher M.
(author)
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Green, Beverley R.
(author)
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Umeå universitetKemiska institutionen
(creator_code:org_t)
Related titles
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In:PLOS ONE: Public Library of Science (PLoS)8:9, s. e74483-1932-6203
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