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Membrane remodeling...
Membrane remodeling by the PX-BAR protein SNX18 promotes autophagosome formation
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Knævelsrud, Helene (author)
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Søreng, Kristiane (author)
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Raiborg, Camilla (author)
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- Håberg, Karin (author)
- Umeå universitet,Institutionen för medicinsk kemi och biofysik
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- Rasmuson, Fredrik (author)
- Umeå universitet,Institutionen för medicinsk kemi och biofysik
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Brech, Andreas (author)
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Liestøl, Knut (author)
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Rusten, Tor Erik (author)
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Stenmark, Harald (author)
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Neufeld, Thomas P (author)
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- Carlsson, Sven R (author)
- Umeå universitet,Institutionen för medicinsk kemi och biofysik
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Simonsen, Anne (author)
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(creator_code:org_t)
- 2013-07-22
- 2013
- English.
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In: Journal of Cell Biology. - : Rockefeller University Press. - 0021-9525 .- 1540-8140. ; 202:2, s. 331-349
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http://jcb.rupress.o...
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https://doi.org/10.1...
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Abstract
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- The membrane remodeling events required for autophagosome biogenesis are still poorly understood. Because PX domain proteins mediate membrane remodeling and trafficking, we conducted an imaging-based siRNA screen for autophagosome formation targeting human PX proteins. The PX-BAR protein SNX18 was identified as a positive regulator of autophagosome formation, and its Drosophila melanogaster homologue SH3PX1 was found to be required for efficient autophagosome formation in the larval fat body. We show that SNX18 is required for recruitment of Atg16L1-positive recycling endosomes to a perinuclear area and for delivery of Atg16L1- and LC3-positive membranes to autophagosome precursors. We identify a direct interaction of SNX18 with LC3 and show that the pro-autophagic activity of SNX18 depends on its membrane binding and tubulation capacity. We also show that the function of SNX18 in membrane tubulation and autophagy is negatively regulated by phosphorylation of S233. We conclude that SNX18 promotes autophagosome formation by virtue of its ability to remodel membranes and provide membrane to forming autophagosomes.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Medicinsk bioteknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Medical Biotechnology (hsv//eng)
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Knævelsrud, Hele ...
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Søreng, Kristian ...
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Raiborg, Camilla
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Håberg, Karin
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Rasmuson, Fredri ...
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Brech, Andreas
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show more...
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Liestøl, Knut
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Rusten, Tor Erik
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Stenmark, Harald
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Neufeld, Thomas ...
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Carlsson, Sven R
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Simonsen, Anne
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show less...
- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Medical Biotechn ...
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and Medical Biotechn ...
- Articles in the publication
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Journal of Cell ...
- By the university
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Umeå University