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Isolation of monome...
Isolation of monomeric photosystem II that retains the subunit PsbS.
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Haniewicz, Patrycja (author)
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De Sanctis, Daniele (author)
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Büchel, Claudia (author)
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- Schröder, Wolfgang P (author)
- Umeå universitet,Kemiska institutionen
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Loi, Maria Cecilia (author)
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- Kieselbach, Thomas (author)
- Umeå universitet,Kemiska institutionen
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Bochtler, Matthias (author)
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Piano, Dario (author)
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(creator_code:org_t)
- 2013-08-24
- 2013
- English.
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In: Photosynthesis Research. - : Springer. - 0166-8595 .- 1573-5079. ; 118:3, s. 199-207
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Abstract
Subject headings
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- Photosystem II has been purified from a transplastomic strain of Nicotiana tabacum according to two different protocols. Using the procedure described in Piano et al. (Photosynth Res 106:221-226, 2010) it was possible to isolate highly active PSII composed of monomers and dimers but depleted in their PsbS protein content. A "milder" procedure than the protocol reported by Fey et al. (Biochim Biophys Acta 1777:1501-1509, 2008) led to almost exclusively monomeric PSII complexes which in part still bind the PsbS protein. This finding might support a role for PSII monomers in higher plants.
Subject headings
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Publication and Content Type
- ref (subject category)
- art (subject category)
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