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Quantification of m...
Quantification of melatonin in human saliva by liquid chromatography-tandem mass spectrometry using stable isotope dilution
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- Eriksson, Kåre (author)
- Umeå universitet,Yrkes- och miljömedicin
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- Östin, Anders (author)
- National Institute for Working Life, Umeå
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- Levin, Jan-Olof (author)
- National Institute for Working Life, Umeå
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(creator_code:org_t)
- Elsevier, 2003
- 2003
- English.
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In: Journal of chromatography. B. - : Elsevier. - 1570-0232 .- 1873-376X. ; 794:1, s. 115-123
- Related links:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
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- A method for the determination of melatonin in human saliva has been developed using high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS-MS). Saliva was collected in plastic tubes. 7-D-Melatonin was added as internal standard and the samples were cleaned and concentrated by solid-phase extraction. The limit of detection was 1.05 pg x ml(-1) and the limit of quantification was 3.0 pg x ml(-1). The accuracy of the method was +/-14% at 5.60 pg x ml(-1) and +/-9% at 19.6 pg x ml(-1). The precision was +/-13% at 6.18 pg x ml(-1) and +/-11% at 31.2 pg x ml(-1), respectively. Our HPLC-MS-MS method shows a high sensitivity and specificity for melatonin and more reliable results compared with a radioimmunoassay. The chromatographic method has been used to determine the circadian rhythm of melatonin among three nurses working the night shift and a patient suffering from an inability to fall asleep at night.
Subject headings
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Keyword
- melatonin
Publication and Content Type
- ref (subject category)
- art (subject category)
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