Arabidopsis SABRE and CLASP interact to stabilize cell division plane orientation and planar polarity

Pietra, Stefano,1980-Umeå universitet,Institutionen för fysiologisk botanik,Umeå Plant Science Centre (UPSC) (author)

The orientation of cell division and the coordination of cell polarity within the plane of the tissue layer (planar polarity) contribute to shape diverse multicellular organisms. The root of Arabidopsis thaliana displays regularly oriented cell divisions, cell elongation and planar polarity providing a plant model system to study these processes. Here we report that the SABRE protein, which shares similarity with proteins of unknown function throughout eukaryotes, has important roles in orienting cell division and planar polarity. SABRE localizes at the plasma membrane, endomembranes, mitotic spindle and cell plate. SABRE stabilizes the orientation of CLASP-labelled preprophase band microtubules predicting the cell division plane, and of cortical microtubules driving cell elongation. During planar polarity establishment, sabre is epistatic to clasp at directing polar membrane domains of Rho-of-plant GTPases. Our findings mechanistically link SABRE to CLASP-dependent microtubule organization, shedding new light on the function of SABRE-related proteins in eukaryotes.

Gustavsson, AnnaUmeå universitet,Institutionen för fysiologisk botanik,Umeå Plant Science Centre (UPSC)(Swepub:umu)angu0056 (author)
Kiefer, ChristianUmeå universitet,Institutionen för fysiologisk botanik,Umeå Plant Science Centre (UPSC)(Swepub:umu)chki0006 (author)
Kalmbach, LotharUmeå universitet,Institutionen för fysiologisk botanik,Umeå Plant Science Centre (UPSC) (author)
Hörstedt, PerUmeå universitet,Institutionen för fysiologisk botanik,Umeå Core Facility Electron Microscopy, Umeå University(Swepub:umu)peho0001 (author)
Ikeda, YoshihisaUmeå universitet,Institutionen för fysiologisk botanik,Umeå Plant Science Centre (UPSC) (author)
Stepanova, Anna N.Department of Genetics, North Carolina State University, Raleigh, North Carolina 27695, USA (author)
Alonso, Jose M.Department of Genetics, North Carolina State University, Raleigh, North Carolina 27695, USA (author)
Grebe, MarkusUmeå universitet,Institutionen för fysiologisk botanik,Umeå Plant Science Centre (UPSC)(Swepub:umu)magr0036 (author)
Umeå universitetInstitutionen för fysiologisk botanik (creator_code:org_t)

By the author/editor: Pietra, Stefano, ...; Gustavsson, Anna; Kiefer, Christia ...; Kalmbach, Lothar; Hörstedt, Per; Ikeda, Yoshihisa; show more...; Stepanova, Anna ...; Alonso, Jose M.; Grebe, Markus; show less...

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