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Ultraviolet illumin...
Ultraviolet illumination-induced reduction of alpha-lactalbumin disulfide bridges
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Permyakov, Eugene A (author)
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Permyakov, Serge E (author)
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Deikus, Gintaras Y (author)
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- Morozova-Roche, Ludmila A (author)
- Umeå universitet,Kemiska institutionen,Institutionen för medicinsk kemi och biofysik
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Grishchenko, Valery M (author)
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Kalinichenko, Lina P (author)
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Uversky, Vladimir N (author)
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(creator_code:org_t)
- 2003-04-09
- 2003
- English.
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In: Proteins: Structure, Function, and Bioinformatics. - : Wiley. - 0887-3585. ; 51:4, s. 498-503
- Related links:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
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- Prolonged exposure of Ca2+-loaded or Ca2+-depleted human -lactalbumin to ultraviolet light (270-290 nm, 1 mW/cm2, for 2 to 4 h) results in a 10-nm red shift of its tryptophan fluorescence spectrum. Gel chromatography of the UV-illuminated samples reveals two non-native protein forms: (1) a component with a red-shifted tryptophan fluorescence spectrum; and (2) a component with kynurenine-like fluorescent properties. The first component has from 0.6 to 0.9 free DTNB-reactive SH groups per protein molecule, which are absent in the native protein and is characterized by slightly lowered Ca2+-affinity (2 × 108 M-1 versus 8 × 108 M-1 for the native protein) and absence of observable thermal transition. The second component corresponds to the protein with photochemically modified tryptophan residues. It is assumed that the UV excitation of tryptophan residue(s) in -lactalbumin is followed by a transfer of electrons to the SS bonds, resulting in their reduction. Mass spectrometry data obtained for trypsin-fragmented UV-illuminated -lactalbumin with acrylodan-modified free thiol groups reveal the reduction of the 61-77 and 73-91 disulfide bridges. The effect observed has to be taken into account in any UV-region spectral studies of -lactalbumin. Proteins 2003;51:498-503.
Keyword
- alpha-lactalbumin
- tryptophan
- cystein
- disulfide bridge
- photo-induced modification
Publication and Content Type
- ref (subject category)
- art (subject category)
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