Some Characteristics of Human Prostasomes and Their Relationship to Prostate Cancer

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Some Characteristics of Human Prostasomes and Their Relationship to Prostate Cancer

Ronquist, Göran, 1971- (author): Uppsala universitet,Klinisk kemi,Institutionen för medicinska vetenskaper,Prostasomforskning (Anders Larsson)

Larsson, Anders, Professor (thesis advisor): Uppsala universitet,Klinisk kemi

Carlsson, Lena, Dr (thesis advisor): Uppsala universitet,Klinisk kemi

Llorente, Alicia, Dr (opponent): Dept. of Biochemistry, Institute for Cancer Research The Norwegian Radium Hospital Montebello

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ISBN 9789155475048
Uppsala : Acta Universitatis Upsaliensis, 2009
English 53 s.
Series: Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Medicine, 1651-6206 ; 451

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Doctoral thesis (other academic/artistic)

Abstract Subject headings

Background: The secretory epithelial cells of the prostate gland use sophisticated vehicles named prostasomes to relay important information to sperm cells in semen. This prostasome-forming and secretory ability of the epithelial cells is also preserved in poorly differentiated prostate cancer cells. Aim: The aim of this thesis was to examine different characteristics of prostasomes, especially those derived from malignant prostate cells, linked to their potential role in diagnosis and prognostication of prostate cancer. Results: Serum samples of prostate cancer patients contained autoantibodies against seminal prostasomes in a higher concentration than did control sera. These autoantibodies were most frequently directed against 25 prostasome-associated proteins, but no one was prostate specific. Clusterin was one of the most frequently occurring prostasomal proteins. Elevated titers were however seen in both patients´ and control sera. Clusterin turned out to be a major antigen of seminal prostasomes. No prostate specific or prostate cancer specific protein was discovered upon proteomic analysis of prostasomes deriving from malignant cells of vertebral metastases of prostate cancer patients. Human chromosomal DNA was identified in both seminal prostasomes and PC-3 cell prostasomes and strong evidence existed that the DNA was localized inside the prostasomes. Four out of 13 DNA clones of seminal prostasomes featured gene sequences (31%). The corresponding figures for PC-3 cell prostasomes were 4 out of 16 clones (25%). Conclusions: Prostasomes are immunogenic and give rise to serum autoantibodies. The most frequently occurring autoantibodies were directed against 25 prostasomal proteins but none of these was exclusively prostate specific. Thirty different proteins were identified in prostate cancer metastasis-derived prostasomes but none of these proteins was prostate cancer specific. Human chromosomal DNA was identified in prostasomes of both normal and malignant cell origin.

Subject headings

MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Annan klinisk medicin (hsv//swe)
MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Other Clinical Medicine (hsv//eng)

Keyword

Prostasomes
prostate
prostate cancer
clusterin
antibodies
metastasis
exosomes
PC-3 cells
DNA vehicles
gene therapy
immunoblotting
mass spectrometry
agarose gel electrophoresis
vertebral metastasis.
Clinical chemistry
Klinisk kemi
Clinical Chemistry
klinisk kemi

Publication and Content Type

vet (subject category)
dok (subject category)

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