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Minimal residual di...
Minimal residual disease assessment in childhood acute lymphoblastic leukemia : Results of a Swedish multi-centre study comparing real-time PCR and multi-colour flow cytometry
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- Thörn, Ingrid, 1957- (author)
- Uppsala universitet,Hematologi och immunologi,Molecular Hematology
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- Forestier, Erik (author)
- Department of Clinical Sciences, Paediatrics, Umeå University, Umeå, Sweden
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- Botling, Johan (author)
- Uppsala universitet,Hematologi och immunologi,Molecular Hematology
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- Thuresson, Britt (author)
- Division of Hematology & Transfusion Medicine, Department of Laboratory Medicine, Lund University, Lund, Sweden
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- Wasslavik, Carina (author)
- Department of Clinical Chemistry and Transfusion Medicine, Sahlgrenska University Hospital, Gothenburg, Sweden
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- Björklund, Elisabeth (author)
- Department of Pathology, Karolinska University Hospital, Stockholm, Sweden
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- Aihong, Li (author)
- Department of Biosciences, Clinical Chemistry, Umeå University, Umeå, Sweden
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- Lindström-Eriksson, Elenor (author)
- Department of Medical Biosciences, Pathology, Umeå University, Umeå, Sweden
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- Grönlund, Elisabet (author)
- Department of Medical Biosciences, Pathology, Umeå University, Umeå, Sweden
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- Torikka, Kerstin (author)
- Division of Hematology & Transfusion Medicine, Department of Laboratory Medicine, Lund University, Lund, Sweden
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- Heldrup, Jesper (author)
- Department of Pediatrics, Lund University Hospital, Lund, Sweden
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- Abrahamsson, Jonas (author)
- Queen Silvias Children’s Hospital, Gothenburg, Sweden
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- Behrendtz, Mikael (author)
- Department of Pediatrics, University Hospital, Linköping, Sweden
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- Söderhäll, Stefan (author)
- Department of Pediatric Oncology, Karolinska University Hospital, Stockholm, Sweden
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- Jacobsson, Stefan (author)
- Department of Clinical Chemistry and Transfusion Medicine, Sahlgrenska University Hospital, Gothenburg, Sweden
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- Roos, Göran (author)
- Department of Medical Biosciences, Pathology, Umeå University, Umeå, Sweden
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- Olofsson, Tor (author)
- Division of Hematology & Transfusion Medicine, Department of Laboratory Medicine, Lund University, Lund, Sweden
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- Porwit, Anna (author)
- Department of Pathology, Karolinska University Hospital, Stockholm, Sweden
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- Lönnerholm, Gudmar (author)
- Uppsala universitet,Institutionen för kvinnors och barns hälsa
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- Rosenquist, Richard (author)
- Uppsala universitet,Hematologi och immunologi,Molecular Hematology
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- Sundström, Christer (author)
- Uppsala universitet,Hematologi och immunologi,Molecular Hematology
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(creator_code:org_t)
- 2009
- English.
- Related links:
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https://urn.kb.se/re...
Abstract
Subject headings
Close
- In this Swedish multi-center study of early treatment response in childhood acute lymphoblastic leukemia (ALL), we evaluated the concordance between multicolour flow cytometry (FCM) and real-time quantitative polymerase chain reaction (RQ-PCR) for assessment of minimal residual disease (MRD). Multiple time points (i.e. day 15, 29, 50 and 106) were evaluated with the NOPHO (Nordic Society of Pediatric Hematology and Oncology) ALL 2000 treatment protocol as backbone. During 2002-2006, 334 children were diagnosed with ALL, where 228 had paired samples taken at any of the four time points. With the detection level of 0.1%, the concordance between RQ-PCR and FCM was 90% in the 726 paired samples analyzed. At day 29, the correlation between the methods was greater with MRD levels >0.1% (rs=0.7, p<0.001) than below (rs=0.2, p=0.024). MRD levels higher than 0.1% at day 29 was a significant predictor of higher risk of having a bone marrow relapse. This was true both for BCP ALL and T-ALL analysed with either FCM or RQ-PCR, although RQ-PCR was a better discriminator than FCM in T-ALL. However, using the NOPHO ALL 2000 protocol, our data indicate that a higher cut-off value (0.2%) should be applied in BCP ALL when using RQ-PCR as MRD method. In contrast, MRD levels ≥ 0.1%, analysed with either method late during induction therapy, was not a predictor of isolated extramedullary relapse. We therefore conclude that MRD assessment by RQ-PCR based IG/TCR rearrangement and multicolour FCM monitoring can be used as a clinical tool if the aim is to find childhood ALL cases with increased risk of having bone marrow relapses.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Keyword
- minimal residual disease
- childhood acute lymphoblastic leukemia
- flow cytometry
- RQ-PCR
- rearranged IG/TCR genes
- Pathology
- Patologi
- Pathology
- Patologi
Publication and Content Type
- vet (subject category)
- ovr (subject category)
To the university's database
- By the author/editor
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Thörn, Ingrid, 1 ...
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Forestier, Erik
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Botling, Johan
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Thuresson, Britt
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Wasslavik, Carin ...
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Björklund, Elisa ...
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show more...
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Aihong, Li
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Lindström-Erikss ...
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Grönlund, Elisab ...
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Torikka, Kerstin
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Heldrup, Jesper
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Abrahamsson, Jon ...
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Behrendtz, Mikae ...
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Söderhäll, Stefa ...
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Jacobsson, Stefa ...
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Roos, Göran
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Olofsson, Tor
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Porwit, Anna
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Lönnerholm, Gudm ...
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Rosenquist, Rich ...
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Sundström, Chris ...
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- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Cell and Molecul ...
- By the university
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Uppsala University