Search: onr:"swepub:oai:DiVA.org:uu-102154" >
Regulation of EphA ...
Regulation of EphA 4 kinase activity is required for a subset of axon guidance decisions suggesting a key role for receptor clustering in Eph function
-
Egea, Joaquim (author)
-
Nissen, Ulla Vig (author)
-
Dufour, Audrey (author)
-
show more...
-
Sahin, Mustafa (author)
-
Greer, Paul (author)
-
- Kullander, Klas (author)
- Uppsala universitet,Institutionen för neurovetenskap,Gen Utvecklingbiologi,Genetisk utvecklingsbiologi
-
Mrsic-Flogel, Thomas D (author)
-
Greenberg, Michael E (author)
-
- Kiehn, Ole (author)
- Karolinska Institutet
-
Vanderhaeghen, Pierre (author)
-
Klein, Rüdiger (author)
-
show less...
-
(creator_code:org_t)
- Elsevier BV, 2005
- 2005
- English.
-
In: Neuron. - : Elsevier BV. - 0896-6273 .- 1097-4199. ; 47:4, s. 515-528
- Related links:
-
http://www.cell.com/...
-
show more...
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
https://urn.kb.se/re...
-
http://kipublication...
-
show less...
Abstract
Subject headings
Close
- Signaling by receptor tyrosine kinases (RTKs) is mediated by their intrinsic kinase activity. Typically, kinase-activating mutations result in ligand-independent signaling and gain-of-function phenotypes. Like other RTKs, Ephs require kinase activity to signal, but signaling by Ephs in vitro also requires clustering by their membrane bound ephrin ligands. The relative importance of Eph kinase activity and clustering for in vivo functions is unknown. We find that knockin mice expressing a mutant form of EphA4 (EphA4(EE)), whose kinase is constitutively activated in the absence of ephrinB ligands, are deficient in the development of thalamocortical projections and some aspects of central pattern generator rhythmicity. Surprisingly, other functions of EphA4 were regulated normally by EphA4(EE), including midline axon guidance, hindlimb locomotion, in vitro growth cone collapse, and phosphorylation of ephexin1. These results suggest that signaling of Eph RTKs follows a multistep process of induced kinase activity and higher-order clustering different from RTKs responding to soluble ligands.
Keyword
- MEDICINE
- MEDICIN
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
-
Neuron
(Search for host publication in LIBRIS)
To the university's database
- By the author/editor
-
Egea, Joaquim
-
Nissen, Ulla Vig
-
Dufour, Audrey
-
Sahin, Mustafa
-
Greer, Paul
-
Kullander, Klas
-
show more...
-
Mrsic-Flogel, Th ...
-
Greenberg, Micha ...
-
Kiehn, Ole
-
Vanderhaeghen, P ...
-
Klein, Rüdiger
-
show less...
- Articles in the publication
-
Neuron
- By the university
-
Uppsala University
-
Karolinska Institutet