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Y chromosomal STR a...
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Edlund, HannaUppsala universitet,Genomik,Medicinsk genetik och genomik
(author)
Y chromosomal STR analysis using Pyrosequencing technology
- Article/chapterEnglish2009
Publisher, publication year, extent ...
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Elsevier BV,2009
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printrdacarrier
Numbers
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LIBRIS-ID:oai:DiVA.org:uu-102854
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https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-102854URI
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https://doi.org/10.1016/j.fsigen.2008.11.010DOI
Supplementary language notes
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Language:English
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Summary in:English
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Classification
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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Analysis of Y chromosome STR markers has proven to be useful in forensic cases where the samples contain a mixture of DNA from several individuals. STR markers are commonly genotyped based on length separation of PCR products. In this study we evaluated if Pyrosequencing can be used as an alternative method for determining Y-STR variants. In total 70 unrelated Swedish males were typed for the Y chromosomal markers (DYS19, DYS389 I-II, DYS390, DYS391, DYS392, DYS393 and DYS438) using Pyrosequencing. Using the 8 markers, 57 unique haplotypes were observed with a discrimination capacity of 0.81. At four loci, the Pyrosequencing analysis revealed sequence variants. The sequence variants were found in the DYS389 II, DYS390, DYS391, and DYS393 loci in frequencies between 1.43% and 14.3%. Pyrosequencing has here been shown to be a useful tool for typing Y chromosomal STRs and the method can provide a complement to conventional forensic Y STR analyses. Moreover, the Pyrosequencing method can be used to rapidly evaluate novel markers.
Subject headings and genre
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Pyrosequencing
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Y chromosome STR
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Forensic identification
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MEDICINE
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MEDICIN
Added entries (persons, corporate bodies, meetings, titles ...)
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Allen, MarieUppsala universitet,Genomik,Medicinsk genetik och genomik(Swepub:uu)marialle
(author)
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Uppsala universitetGenomik
(creator_code:org_t)
Related titles
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In:Forensic Science International: Elsevier BV3:2, s. 119-1241872-49731878-0326
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