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Quantitative real-time PCR analysis and microarray-based RNA expression of HER2 in relation to outcome
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- Bergqvist, Jenny (author)
- Karolinska Institutet
- Ohd, J. F. (author)
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- Smeds, J. (author)
- Karolinska Institutet
- show more...
- Klaar, Sigrid (author)
- Isola, J. (author)
- Elmberger, G. P. (author)
- Hellborg, H. (author)
- Borg, A-L. (author)
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- (creator_code:org_t)
- Elsevier BV, 2007
- 2007
- English.
- In: Annals of Oncology. - : Elsevier BV. - 0923-7534 .- 1569-8041. ; 18:5, s. 845-850
- Journal article (peer-reviewed)
Abstract
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- BACKGROUND: Our aim was to use quantitative real-time PCR (Q-PCR) and RNA expression profiles (RNA-EPs) to investigate HER2 status in relation to outcome. PATIENTS AND METHODS: Cut-off levels for Q-PCR and RNA-EP were established in relation to immunohistochemistry (IHC) validated by FISH in a test set of frozen tissue samples from 40 primary breast cancers. The HER2 status was subsequently studied in another validation set of 306 tumors, where Q-PCR and RNA-EP results were compared with previously carried out IHC that we had validated by chromogenic in situ hybridization (CISH). RESULTS: Q-PCR and RNA-EP offered similar sensitivity (90% versus 77%), specificity (93% versus 95%), and negative (99% versus 98%) and positive (63% versus 61%) predictive values for HER2 determinations. Analyses of relapse-free survival (RFS) and overall survival on the basis of 5 and 10 years of follow-up indicated equivalent hazard ratios for all three techniques. In contrast to IHC/CISH, both Q-PCR and RNA-EP analyses of HER2 also gave statistically significant results regarding RFS and breast cancer-corrected survival after 10 years of follow-up. CONCLUSION: The use of RNA-EP and Q-PCR to analyze HER2 in frozen and formalin-fixed breast cancer samples may be an alternate approach to IHC in combination with FISH/CISH.
Keyword
- breast cancer
- CISH
- diagnostic methods
- HER2
- quantitative real-time PCR
- RNA expression profiles
- MEDICINE
- MEDICIN
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- ref (subject category)
- art (subject category)
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- Bergqvist, Jenny
- Ohd, J. F.
- Smeds, J.
- Klaar, Sigrid
- Isola, J.
- Nordgren, Hans
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- Elmberger, G. P.
- Hellborg, H.
- Bjohle, J.
- Borg, A-L.
- Skoog, L.
- Bergh, J.
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- Uppsala University
- Karolinska Institutet
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