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Prolonged antigen-e...
Prolonged antigen-exposure with carbohydrate particle based vaccination prevents allergic immune responses in sensitized mice
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- Thunberg, Sarah, 1976- (author)
- Karolinska Institutet
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- Neimert-Andersson, T. (author)
- Karolinska Institutet
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- Cheng, Q. (author)
- Karolinska Institutet
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- Wermeling, F. (author)
- Karolinska Institutet
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- Bergström, U. (author)
- Uppsala universitet,Ekotoxikologi,Uppsala university, Sweden
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- Swedin, L. (author)
- Karolinska Institutet, Sweden
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- Dahlén, S.-E. (author)
- Karolinska Institutet
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- Arnér, E. (author)
- Karolinska Institutet
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- Scheynius, A. (author)
- Karolinska Institutet
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- Karlsson, M. C. I. (author)
- Karolinska Institutet
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- Gafvelin, G. (author)
- Karolinska Institutet
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- van Hage, M (author)
- Karolinska Institutet
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- Grönlund, H. (author)
- Karolinska Institutet
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(creator_code:org_t)
- Wiley, 2009
- 2009
- English.
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In: Allergy. European Journal of Allergy and Clinical Immunology. - : Wiley. - 0105-4538 .- 1398-9995. ; 64:6, s. 919-926
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Abstract
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- BACKGROUND: Defined particles carrying tightly bound allergens at high density have been suggested as alternatives in allergy vaccination. Carbohydrate based particles (CBP), sized 2 microm, provide a platform for covalent coupling of allergens. OBJECTIVE: To investigate the mechanisms of antigen presentation by CBP, as well as cellular and humoral responses after vaccination with the major cat allergen Fel d 1, covalently coupled to CBP. METHODS: Mice (n = 10/group) were subcutaneously vaccinated with CBP-rFel d 1, CBP or phosphate buffer saline (PBS) before sensitization with rFel d 1 and challenged with cat dander extract. Fluorescent and (75)Se-radiolabeled tracking of allergens and particles were performed with flow cytometry and whole-body autoradiography. Humoral, cellular and regulatory immune responses were analyzed by ELISA and flow cytometry. Cytokines were measured in bronchoalveolar lavage fluid and splenocyte cultures. RESULTS: CBP-rFel d 1 prevented induction of airway inflammation and induced allergen-specific T-cell anergy. CBP-rFel d 1 also induced rapid IgM and IgG1-responses compared with soluble rFel d 1. Particles were phagocytosed by antigen-presenting cells and transported to draining lymph nodes and spleen. Moreover, antigen coupled to CBP remained longer at the injection site compared with alum. CONCLUSIONS: Covalent coupling of rFel d 1 to CBP induces rapid antibody production, prevents induction of allergic immune responses and systemic allergen spreading. Thus, CBP comprise several attractive adjuvant features for use in allergy vaccination. CLINICAL IMPLICATIONS: Prolonged allergen exposure through covalent coupling to particles suitable for phagocytosis, provides an adjuvant for safer and efficient allergy vaccination.
Subject headings
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine (hsv//eng)
Keyword
- adjuvant
- allergy
- particles
- rFel d 1
- vaccination
- Biology
- Biologi
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Thunberg, Sarah, ...
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Neimert-Andersso ...
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Cheng, Q.
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Wermeling, F.
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Bergström, U.
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Swedin, L.
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show more...
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Dahlén, S.-E.
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Arnér, E.
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Scheynius, A.
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Karlsson, M. C. ...
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Gafvelin, G.
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van Hage, M
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Grönlund, H.
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show less...
- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
- Articles in the publication
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Allergy. Europea ...
- By the university
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Uppsala University
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Royal Institute of Technology
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Karolinska Institutet