Rescue and characterization of episomally replicating DNA from the moss Physcomitrella

Murén, EvaUppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi (author)

The moss Physcomitrella is unique among plants in that it permits efficient gene targeting by homologous recombination. Furthermore, transformed DNA can replicate episomally in Physcomitrella. Here we show that episomally replicating DNA can berescued back into E. coli, and use such rescue to study the fate of the transformed DNA. Significantly, plasmids rescued from moss transformed with circular DNA are identical to the original plasmid, whereas plasmids rescued from moss transformed with linearized DNA frequently have deletions created by direct repeat recombination.These events are highly predictable in that they target the longest direct repeat on the plasmid, if this repeat is at least 12 bp. Episomal transformants obtained with linearized DNA show a more than 1000-fold amplification of the DNA whereas transformants obtained with circular DNA have much lower copy numbers. Most episomal transformants quickly lose the plasmid in the absence of selection, but a semi-stable type of transformant that loses the plasmid at a much lower frequency was also observed. The consistent rescue of the original plasmid, or of predictable derivatives thereof, suggests that molecular genetics methods which rely on shuttle plasmids are feasible in Physcomitrella

Nilsson, AndersUppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi(Swepub:uu)annil103 (author)
Ulfstedt, MikaelUppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi (author)
Johansson, MonikaUppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi(Swepub:uu)monjo282 (author)
Ronne, HansUppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi(Swepub:uu)haron677 (author)
Uppsala universitetInstitutionen för medicinsk biokemi och mikrobiologi (creator_code:org_t)

In:Proceedings of the National Academy of Sciences of the United States of America: Proceedings of the National Academy of Sciences106:46, s. 19444-194490027-84241091-6490

By the author/editor: Murén, Eva; Nilsson, Anders; Ulfstedt, Mikael; Johansson, Monik ...; Ronne, Hans

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