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Printing protein arrays from DNA arrays
- He, Mingyue (author)
- Stoevesandt, Oda (author)
- Palmer, Elizabeth A. (author)
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- Khan, Farid (author)
- Taussig, Michael J. (author)
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- (creator_code:org_t)
- 2008
- 2008
- English.
- In: Nature Methods. - 1548-7091 .- 1548-7105. ; 5:2, s. 175-177
- Journal article (peer-reviewed)
Abstract
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- We describe a method, DNA array to protein array (DAPA), which allows the 'printing' of replicate protein arrays directly from a DNA array template using cell-free protein synthesis. At least 20 copies of a protein array can be obtained from a single DNA array. DAPA eliminates the need for separate protein expression, purification and spotting, and also overcomes the problem of long-term functional storage of surface-bound proteins.
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