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In Vitro Neurotoxic...
In Vitro Neurotoxicity of PBDE-99 : Immediate and Concentration-Dependent Effects on Protein Expression in Cerebral Cortex Cells
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- Alm, Henrik (author)
- Uppsala universitet,Institutionen för farmaceutisk biovetenskap
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- Scholz, Birger (author)
- Uppsala universitet,Institutionen för farmaceutisk biovetenskap
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- Kultima, Kim (author)
- Uppsala universitet,Institutionen för farmaceutisk biovetenskap
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- Nilsson, Anna (author)
- Uppsala universitet,Institutionen för farmaceutisk biovetenskap
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- Andrén, Per E (author)
- Uppsala universitet,Institutionen för farmaceutisk biovetenskap
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- Savitski, Mikhail M (author)
- Uppsala universitet,Institutionen för cell- och molekylärbiologi
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- Bergman, Åke (author)
- Stockholms universitet,Institutionen för material- och miljökemi (MMK)
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- Stigson, Michael (author)
- Uppsala universitet,Institutionen för farmaceutisk biovetenskap
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- Fex-Svenningsen, Åsa (author)
- Institute of Medical Biology, Anatomy and Neurobiology, University of Southern Denmark, Denmark
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- Dencker, Lennart (author)
- Uppsala universitet,Institutionen för farmaceutisk biovetenskap
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(creator_code:org_t)
- 2009-12-22
- 2010
- English.
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In: Journal of Proteome Research. - : American Chemical Society (ACS). - 1535-3893 .- 1535-3907. ; 9:3, s. 1226-1235
- Related links:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
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- Polybrominated diphenyl ethers (PBDEs) are commonly used flame retardants in various consumer products. Pre- and postnatal exposure to congeners of PBDEs disrupts normal brain development in rodents. Two-dimensional difference gel electrophoresis (2D-DIGE) was used to analyze concentration-dependent differences in protein expression in cultured cortical cells isolated from rat fetuses (GD 21) after 24 h exposure to PBDE-99 (3, 10, or 30 muM). Changes on a post-translational level were studied using a 1 h exposure to 30 muM PBDE-99. The effects of 24 h exposure to 3 and 30 muM PBDE-99 on mRNA levels were measured using oligonucleotide microarrays. A total of 62, 46, and 443 proteins were differentially expressed compared to controls after 24 h of exposure to 3, 10, and 30 muM PDBE-99, respectively. Of these, 48, 43, and 238 proteins were successfully identified, respectively. We propose that the biological effects of low-concentration PBDE-99 exposure are fundamentally different than effects of high-concentration exposure. Low-dose PBDE-99 exposure induced marked effects on cytoskeletal proteins, which was not correlated to cytotoxicity or major morphological effects, suggesting that other more regulatory aspects of cytoskeletal functions may be affected. Interestingly, 0.3 and 3 muM, but not 10 or 30 muM increased the expression of phosphorylated (active) Gap43, perhaps reflecting effects on neurite extension processes.
Subject headings
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
- NATURVETENSKAP -- Kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences (hsv//eng)
Keyword
- PBDE
- PBDE-99
- brominated flameretardant
- neurotoxicity
- developmental toxicity
- proteomics
- dose response
- toxicology
- functional genomics
- cytotoxicity
- in vitro
- primary cell culture
- cytoskeleton
- Gap-43
- Biology
- Biologi
- Chemistry
- Kemi
- Toxicology
- Toxikologi
- Biologi med inriktning mot ekotoxikologi
- Biology with specialization in Environmental Toxicology
- Medicinsk utvecklings- och neurobiologi
- Developmental Neurosciences
- NATURAL SCIENCES
Publication and Content Type
- ref (subject category)
- art (subject category)
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To the university's database
- By the author/editor
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Alm, Henrik
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Scholz, Birger
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Kultima, Kim
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Nilsson, Anna
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Andrén, Per E
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Savitski, Mikhai ...
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show more...
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Bergman, Åke
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Stigson, Michael
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Fex-Svenningsen, ...
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Dencker, Lennart
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show less...
- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Chemical Science ...
- Articles in the publication
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Journal of Prote ...
- By the university
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Uppsala University
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Stockholm University