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Gene expression and...
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Banduseela, Varuna C.Uppsala universitet,Institutionen för neurovetenskap,neurofysiologi
(author)
Gene expression and muscle fiber function in a porcine ICU model
- Article/chapterEnglish2009
Publisher, publication year, extent ...
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American Physiological Society,2009
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printrdacarrier
Numbers
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LIBRIS-ID:oai:DiVA.org:uu-120652
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https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-120652URI
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https://doi.org/10.1152/physiolgenomics.00026.2009DOI
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http://kipublications.ki.se/Default.aspx?queryparsed=id:119587435URI
Supplementary language notes
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Language:English
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Summary in:English
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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Skeletal muscle wasting and impaired muscle function in response to mechanical ventilation and immobilization in intensive care unit (ICU) patients are clinically challenging partly due to 1) the poorly understood intricate cellular and molecular networks and 2) the unavailability of an animal model mimicking this condition. By employing a unique porcine model mimicking the conditions in the ICU with long-term mechanical ventilation and immobilization, we have analyzed the expression profile of skeletal muscle biopsies taken at three time points during a 5-day period. Among the differentially regulated transcripts, extracellular matrix, energy metabolism, sarcomeric and LIM protein mRNA levels were downregulated, while ubiquitin proteasome system, cathepsins, oxidative stress responsive genes and heat shock proteins (HSP) mRNAs were upregulated. Despite 5 days of immobilization and mechanical ventilation single muscle fiber cross-sectional areas as well as the maximum force generating capacity at the single muscle fiber level were preserved. It is proposed that HSP induction in skeletal muscle is an inherent, primary, but temporary protective mechanism against protein degradation. To our knowledge, this is the first study that isolates the effect of immobilization and mechanical ventilation in an ICU condition from various other cofactors.
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Ochala, JulienUppsala universitet,Institutionen för neurovetenskap,neurofysiologi(Swepub:uu)juloc433
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Chen, Yi-Wen
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Göransson, HannaUppsala universitet,Institutionen för medicinska vetenskaper,Cancer Pharmacology and Informatics(Swepub:uu)hagor102
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Norman, HollyUppsala universitet,Institutionen för neurovetenskap,neurofysiologi
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Radell, PeterKarolinska Institutet
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Eriksson, Lars I.Karolinska Institutet
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Hoffman, Eric P.
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Larsson, LarsUppsala universitet,Institutionen för neurovetenskap,neurofysiologi(Swepub:uu)lalar021
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Uppsala universitetInstitutionen för neurovetenskap
(creator_code:org_t)
Related titles
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In:Physiological Genomics: American Physiological Society39:3, s. 141-1591094-83411531-2267
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