Labelling of polysaccharides using [11C]cyanogen bromide: In vivo and in vitro evaluation of 11C-hyaluronan uptake kinetics

Labelling of polysaccharides using [11C]cyanogen bromide : In vivo and in vitro evaluation of 11C-hyaluronan uptake kinetics

A method for the 11C-labelling of polysaccharides in high specific radioactivity is described. Dextran and hyaluronan were treated with [11C]cyanogen bromide in aqueous solution at pH 11.5 to give 30-47% radiochemical yields with higher than 98% radiochemical purity in synthesis times of 24-26 min counted from the end of bombardment. Specific radioactivities at the end of synthesis ranged from 0.12 to 3.1 Ci/mumol. The biodistribution kinetics of [11C]hyaluronan injected intravenously was studied in rats by means of positron emission tomography, showing a rapid and displaceable uptake in liver. Uptake and displacement of [11C]hyaluronan was also demonstrated in cultured rat liver endothelial cells.

Bergström, Mats (author)
Gustafson, Stefan (author)
Lindqvist, UllaUppsala universitet,Institutionen för medicinska vetenskaper,Reumatologi (author)
Sundin, AndersUppsala universitet,Enheten för radiologi(Swepub:uu)anderssu (author)
Långström, Bengt (author)
Uppsala universitetInstitutionen för medicinska vetenskaper (creator_code:org_t)

By the author/editor: Westerberg, Göra ...; Bergström, Mats; Gustafson, Stefa ...; Lindqvist, Ulla; Sundin, Anders; Långström, Bengt

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.