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Quantitative PCR an...
Quantitative PCR analysis of fungal DNA in Swedish day care centers and comparison with building characteristics and allergen levels
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- Cai, G-H (author)
- Uppsala universitet,Arbets- och miljömedicin
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- Bröms, Kristina (author)
- Uppsala universitet,Allmänmedicin och klinisk epidemiologi
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Mälarstig, B (author)
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- Zhao, Z-H (author)
- Uppsala universitet,Arbets- och miljömedicin,eva vingård
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- Kim, Jeong-Lim (author)
- Gothenburg University,Göteborgs universitet,Uppsala universitet,Arbets- och miljömedicin,eva vingård,Institutionen för medicin, avdelningen för samhällsmedicin och folkhälsa,Institute of Medicine, School of Public Health and Community Medicine
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- Svärdsudd, Kurt (author)
- Uppsala universitet,Allmänmedicin och klinisk epidemiologi
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- Janson, Christer (author)
- Uppsala universitet,Lungmedicin och allergologi
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- Norbäck, Dan (author)
- Uppsala universitet,Arbets- och miljömedicin
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(creator_code:org_t)
- Hindawi Limited, 2009
- 2009
- English.
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In: Indoor Air. - : Hindawi Limited. - 0905-6947 .- 1600-0668. ; 19:5, s. 392-400
- Related links:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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https://gup.ub.gu.se...
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Abstract
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- Sweden has had allergen-avoidance day care centers (AADCs) since 1979. The aim of this study was to measure fungal DNA by quantitative polymerase chain reaction (qPCR), a new method, in AADCs and ordinary day care centers (ODCs) and examine associations between allergen levels and building characteristics. Dust samples were collected by swabbing doorframes, vacuum-cleaning, and using Petri dishes. In total, 11 AADCs and 11 ODCs were studied (70 rooms). Total fungal DNA, measured by qPCR in the swab dust, was detected in 89%, Aspergillus or Penicillium (Asp/Pen) DNA in 34%, and Stachybotrys chartarum DNA in 6% of the rooms. Total fungal DNA was significantly higher in rooms with linoleum floor (P = 0.02), textile carpets (P = 0.03), reported dampness/molds (P = 0.02) and reported odor (P < 0.001) in the buildings, and significantly lower in wooden facade buildings (P = 0.003). Reported odor was related to the amount of sieved fine dust, reported dampness/molds and type of building construction. Total fungal DNA was related to cat, dog, horse and total allergen levels (P = 0.003) in the day care centers. In conclusion, total fungal DNA is related to reported dampness/molds, reported odor, and type of wall construction. The association between fungal and allergen contamination indicated a general 'hygiene factor' related to biological contaminants. Practical Implications The associations between fungal DNA, reported dampness/molds, and odor support the view that buildings with odor problems should be investigated for possible hidden fungal growth. There is a need to measure fungal biomass in different types of building constructions by monitoring fungal DNA. Analysis of fungal DNA with quantitative PCR can be a fast and practical way to study indoor fungal contamination. Swabbing dust from the doorframe of the main entrance to the room can be a convenient method of sampling dust for fungal DNA analysis. The high prevalence of reported dampness/molds and the common occurrence of fungal DNA indicate the need to improve the indoor environment of Swedish day care centers.
Subject headings
- SAMHÄLLSVETENSKAP -- Annan samhällsvetenskap -- Övrig annan samhällsvetenskap (hsv//swe)
- SOCIAL SCIENCES -- Other Social Sciences -- Other Social Sciences not elsewhere specified (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Mikrobiologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Microbiology in the medical area (hsv//eng)
Keyword
- Day care centers
- Quantitative PCR
- Fungal DNA
- Allergen levels
- Indoor environment
- Building dampness
- MEDICINE
- MEDICIN
Publication and Content Type
- ref (subject category)
- art (subject category)
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