Search: onr:"swepub:oai:DiVA.org:uu-124921" >
Use of a commercial...
Use of a commercial line blot assay as a screening test for autoantibodies in inflammatory myopathies
-
- Rönnelid, Johan (author)
- Uppsala universitet,Enheten för klinisk immunologi,Johan Rönnelid
-
- Barbasso Helmers, Sevim (author)
- Karolinska Institutet
-
- Storfors, Helena (author)
- Uppsala universitet,Enheten för klinisk immunologi
-
show more...
-
- Grip, Katarina (author)
- Uppsala universitet,Enheten för klinisk immunologi
-
- Rönnblom, Lars (author)
- Uppsala universitet,Institutionen för medicinska vetenskaper
-
- Franck-Larsson, Karin (author)
- Uppsala universitet,Institutionen för medicinska vetenskaper
-
- Nordmark, Gunnel (author)
- Uppsala universitet,Institutionen för medicinska vetenskaper
-
- Lundberg, Iingrid E. (author)
- Karolinska Institutet
-
show less...
-
(creator_code:org_t)
- Elsevier BV, 2009
- 2009
- English.
-
In: Autoimmunity Reviews. - : Elsevier BV. - 1568-9972 .- 1873-0183. ; 9:1, s. 58-61
- Related links:
-
https://urn.kb.se/re...
-
show more...
-
https://doi.org/10.1...
-
http://kipublication...
-
show less...
Abstract
Subject headings
Close
- AIMS: To evaluate the clinical utility of a commercial immunoblot assay for the detection of myositis-specific autoantibodies. METHODS: Serum samples from 153 myositis patients and 77 disease controls were investigated. The commercial Euroline assay with seven autoantigens (Mi-2, Ku, PM-Scl, Jo-1, Pl-7, Pl-12 and SSA/Ro-52) was used according to the manufacturer s instructions, and supplemented with an anti-SRP strip. In a separate experiment analyses were performed at different temperatures. Results were recorded with densitometry. RESULTS: Anti-Jo-1 was found in 18 myositis and one systemic sclerosis patient. Antibodies against Mi-2 were found in 5 myositis patients, and eleven myositis patients had antibodies against PM-Scl. Four myositis patients showed anti-Pl-7 reactivity, whereas no patients had antibodies against Pl-12. Anti-Ku antibodies were found in 4 myositis and 2 primary Sjögren's syndrome patients. Anti-SRP was found in 8 myositis patients as well as in two disease controls. Antibodies against SSA/Ro52 ranged between 23-62% in all groups except juvenile dermatomyositis patients. Most autoantibody reactivities were clearly positive, only 11% (14/127) were borderline positive. Higher assay temperature increased antibody reactivities. CONCLUSIONS: Except for anti-SSA/Ro-52 and anti-Ku the antibody reactivities were rather myositis-specific, supporting the use of this immunoblot assay. However, assay validation needs to be determined against other methods.
Keyword
- Autoantibodies
- Dermatomyositis
- Polymyositis
- Diagnosis
- MEDICINE
- MEDICIN
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
To the university's database