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pH measurement as q...
pH measurement as quality control on human post mortem brain tissue : a study of the BrainNet Europe consortium
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Monoranu, Camelia Maria (author)
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Apfelbacher, M. (author)
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Grünblatt, E. (author)
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Puppe, B. (author)
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- Alafuzoff, Irina (author)
- Department of Neuroscience and Neurology, University of Kuopio Finland
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Ferrer, I. (author)
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Al-Saraj, S. (author)
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Keyvani, K. (author)
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Schmitt, A. (author)
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Falkai, P. (author)
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Schittenhelm, J. (author)
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Halliday, G. (author)
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Kril, J. (author)
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Harper, C. (author)
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McLean, C. (author)
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Riederer, P. (author)
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Roggendorf, W. (author)
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(creator_code:org_t)
- Wiley, 2009
- 2009
- English.
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In: Neuropathology and Applied Neurobiology. - : Wiley. - 0305-1846 .- 1365-2990. ; 35:3, s. 329-337
- Related links:
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https://europepmc.or...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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https://urn.kb.se/re...
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Abstract
Subject headings
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- AIMS: Most brain diseases are complex entities. Although animal models or cell culture experiments mimic some disease aspects, human post mortem brain tissue remains essential to advance our understanding of brain diseases using biochemical and molecular techniques. Post mortem artefacts must be properly understood, standardized, and either eliminated or factored into such experiments. Here we examine the influence of several premortem and post mortem factors on pH, and discuss the role of pH as a biochemical marker for brain tissue quality. METHODS: We assessed brain tissue pH in 339 samples from 116 brains provided by 8 different European and 2 Australian brain bank centres. We correlated brain pH with tissue source, post mortem delay, age, gender, freezing method, storage duration, agonal state and brain ischaemia. RESULTS: Our results revealed that only prolonged agonal state and ischaemic brain damage influenced brain tissue pH next to repeated freeze/thaw cycles. CONCLUSIONS: pH measurement in brain tissue is a good indicator of premortem events in brain tissue and it signals limitations for post mortem investigations.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Klinisk laboratoriemedicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Clinical Laboratory Medicine (hsv//eng)
Keyword
- agonal state
- brain banking
- brain ischaemia
- human post mortem brain tissue
- pH
- Pathology
- Patologi
- Pathology
- Patologi
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Monoranu, Cameli ...
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Apfelbacher, M.
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Grünblatt, E.
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Puppe, B.
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Alafuzoff, Irina
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Ferrer, I.
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show more...
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Al-Saraj, S.
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Keyvani, K.
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Schmitt, A.
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Falkai, P.
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Schittenhelm, J.
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Halliday, G.
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Kril, J.
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Harper, C.
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McLean, C.
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Riederer, P.
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Roggendorf, W.
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show less...
- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Cell and Molecul ...
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Clinical Medicin ...
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and Clinical Laborat ...
- Articles in the publication
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Neuropathology a ...
- By the university
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Uppsala University