Search: onr:"swepub:oai:DiVA.org:uu-125861" >
Immuno-PCR-based qu...
Immuno-PCR-based quantification of multiple phosphorylated tau-epitopes linked to Alzheimer's disease
-
Singer, David (author)
-
Soininen, Hilkka (author)
-
- Alafuzoff, Irina (author)
- Department of Neuroscience and Neurology, University of Kuopio Finland,Alafuzoff
-
show more...
-
Hoffmann, Ralf (author)
-
show less...
-
(creator_code:org_t)
- 2009-10-11
- 2009
- English.
-
In: Analytical and Bioanalytical Chemistry. - : Springer Science and Business Media LLC. - 1618-2642 .- 1618-2650. ; 395:7, s. 2263-2267
- Related links:
-
https://urn.kb.se/re...
-
show more...
-
https://doi.org/10.1...
-
show less...
Abstract
Subject headings
Close
- Several lines of evidence suggest that quantification of phosphorylated sites in the tau-protein (phospho-tau) might be favorable for early and specific Alzheimer's disease diagnosis. The typical setup to quantify phosphorylated tau-epitopes relies on a sandwich ELISA with a capture antibody (Ab) recognizing tau independent of its phosphorylation status and a detector Ab binding specifically to a certain phosphorylation site. Besides Ab specificities, major challenges arise from the very low tau-concentrations in cerebrospinal fluid (CSF) ranging from 100 to 2,000 pg/ml. Based on the phosphorylation degree of a given position, which can be below 10%, the corresponding phospho-tau-level might be much lower, especially for multiphosphorylated epitopes studied here. Thus, a novel, highly sensitive, and generally applicable immunoassay is described to quantify tau-versions, which are phosphorylated at pThr212/pSer214/pThr231/pSer235, down to tau-concentrations of 2 pg/ml in CSF.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Neurologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Neurology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Keyword
- Alzheimer's disease
- Neurology
- Neurologi
- Pathology
- Patologi
- Neurology
- Neurologi
- Pathology
- Patologi
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
To the university's database