Steroid profiles in ovarian follicular fluid in women with and without polycystic ovary syndrome, analyzed by liquid chromatography-tandem mass spectrometry.

↓ Direkt till sidans innehåll
↓ Direkt till sidans sekundära innehåll (sidomenyn)

Search: onr:"swepub:oai:DiVA.org:uu-126541" > Steroid profiles in...

1 of 1
Previous record
Next record
To hitlist

Details
MARC

Naessén, TordUppsala universitet,Institutionen för kvinnors och barns hälsa,Gynekologisk endokrinologi/Naessén (author)

Steroid profiles in ovarian follicular fluid in women with and without polycystic ovary syndrome, analyzed by liquid chromatography-tandem mass spectrometry.

Article/chapterEnglish2010

Publisher, publication year, extent ...

Elsevier BV,2010
printrdacarrier

Numbers

LIBRIS-ID:oai:DiVA.org:uu-126541
https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-126541URI
https://doi.org/10.1016/j.fertnstert.2009.12.081DOI
http://kipublications.ki.se/Default.aspx?queryparsed=id:121765719URI

Supplementary language notes

Language:English
Summary in:English

Part of subdatabase

SwePubSwePub

Classification

Subject category:ref swepub-contenttype
Subject category:art swepub-publicationtype

Notes

Published online 19 February 2010
OBJECTIVE To compare steroid concentrations and steroid product-to-precursor ratios in ovarian follicular fluid (FF) from women with polycystic ovary syndrome (PCOS) and from regularly menstruating women in their early follicular phase, using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Polycystic ovary syndrome involves abnormal regulation of the steroidogenic enzymes, leading to arrest of follicle development. DESIGN Case-control study. SETTING University hospital clinic. PATIENT(S) Follicular fluid from size-matched ovarian follicles (5-8 mm) in 27 nonstimulated women with PCOS and in 21 women without PCOS was sampled. Thirteen steroids were quantitated from 40 muL of FF, using LC-MS/MS. INTERVENTION(S) None. MAIN OUTCOME MEASURE(S) Concentrations of steroids in the FF and product-to-precursor ratios (enzyme activity) were compared between the groups. RESULT(S) In women with PCOS, ovarian FF contained higher concentrations of individual and total androgens, lower individual and total estrogens (E), and a lower total E-to-androgen ratio, compared with regularly menstruating women. The product-to-precursor concentration ratios indicated higher CYP17-linked and lower CYP19-linked (aromatase) enzyme activity. Receiver operating characteristic plots indicated the early CYP17 step (17-OH5P/5P) being highly important for the prevalence of PCOS (c = 0.95). CONCLUSION(S) The women with PCOS had higher ovarian CYP17-linked and lower CYP19-linked (aromatase) enzyme activity, confirming previous data. Multiple steroid assessments from minute volumes including FF from nonstimulated ovaries, using LC-MS/MS, might be useful in research, clinical endocrinology, and in IVF.

Subject headings and genre

MEDICIN OCH HÄLSOVETENSKAP Klinisk medicin Reproduktionsmedicin och gynekologi hsv//swe
MEDICAL AND HEALTH SCIENCES Clinical Medicine Obstetrics, Gynaecology and Reproductive Medicine hsv//eng
MEDICIN OCH HÄLSOVETENSKAP Medicinska och farmaceutiska grundvetenskaper Andra medicinska och farmaceutiska grundvetenskaper hsv//swe
MEDICAL AND HEALTH SCIENCES Basic Medicine Other Basic Medicine hsv//eng
Androgens
CYP17
CYP19
estrogens
follicular fluid
LC-MS/MS
mass spectrometry
PCOS
Polycystic ovary syndrome
steroid hormones
TECHNOLOGY
TEKNIKVETENSKAP
Obstetrics and women's diseases
Obstetrik och kvinnosjukdomar
Chemistry
Kemi

Added entries (persons, corporate bodies, meetings, titles ...)

Kushnir, Mark M.Uppsala universitet,Analytisk kemi (author)
Chaika, AndreyDonetsk State Medical University, Ukraine (author)
Nosenko, JelenaDonetsk State Medical University, Ukraine (author)
Mogilevkina, IrynaDonetsk State Medical University, Ukraine (author)
Rockwood, Alan L.ARUP Institute for Clinical and Experimental Pathology, Utah (author)
Carlström, KjellKarolinska Institute (author)
Bergquist, JonasUppsala universitet,Analytisk kemi(Swepub:uu)jbe25356 (author)
Kirilovas, DmitrijusUppsala universitet,Institutionen för kvinnors och barns hälsa,Gynekologisk endokrinologi/Naessén(Swepub:uu)dmitkiri (author)
Uppsala universitetInstitutionen för kvinnors och barns hälsa (creator_code:org_t)

Related titles

In:Fertility and Sterility: Elsevier BV94:6, s. 2228-22330015-02821556-5653

Internet link

Find in a library

Fertility and Sterility (Search for host publication in LIBRIS)

To the university's database

1 of 1
Previous record
Next record
To hitlist

Find more in SwePub

By the author/editor: Naessén, Tord; Kushnir, Mark M.; Chaika, Andrey; Nosenko, Jelena; Mogilevkina, Iry ...; Rockwood, Alan L ...; show more...; Carlström, Kjell; Bergquist, Jonas; Kirilovas, Dmitr ...; show less...

About the subject

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Clinical Medicin ...; and Obstetrics Gynae ...

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Basic Medicine; and Other Basic Medi ...

Articles in the publication: Fertility and St ...

By the university: Uppsala University; Karolinska Institutet

Search outside SwePub

Extend your search to:: Google; Google Book Search; Google Scholar

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

LIBRIS.kb.se