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Expression, crystal...
Expression, crystallization and preliminary crystallographic data analysis of filamin A repeats 14-16
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- Aguda, Adeleke Halilu (author)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi
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- Sakwe, Amos Malle (author)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi
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- Rask, Lars (author)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi
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- Robinson, Robert C. (author)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi
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(creator_code:org_t)
- 2007
- 2007
- English.
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In: Acta Crystallographica. Section F. - 1744-3091 .- 1744-3091. ; 63:4, s. 291-293
- Related links:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
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- Human filamin A is a 280 kDa protein involved in actin-filament cross-linking. It is structurally divided into an actin-binding headpiece (ABD) and a rod domain containing 24 immunoglobulin-like (Ig) repeats. A fragment of human filamin A (Ig repeats 14-16) was cloned and expressed in Escherichia coli and the purified protein was crystallized in 1.6 M ammonium sulfate, 2% PEG 1000 and 100 mM HEPES pH 7.5. The crystals diffracted to 1.95 A and belong to space group P2(1)2(1)2(1), with unit-cell parameters a = 50.63, b = 52.10, c = 98.46 A, alpha = beta = gamma = 90 degrees.
Keyword
- ABP-280
- Calponin homology
- Cytoskeleton
- Filamin
- MEDICINE
- MEDICIN
Publication and Content Type
- ref (subject category)
- art (subject category)
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