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Colorimetric Protei...
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Aili, Daniel,1977-Linköpings universitet,Molekylär fysik,Tekniska fakulteten
(author)
Colorimetric Protein Sensing by Controlled Assembly of Gold Nanoparticles Functionalized with Synthetic Receptors
- Article/chapterEnglish2009
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Wiley,2009
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Numbers
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LIBRIS-ID:oai:DiVA.org:uu-127413
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https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-127413URI
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https://doi.org/10.1002/smll.200900530DOI
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https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-15122URI
Supplementary language notes
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Language:English
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Summary in:English
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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A novel strategy is described for the colorimetric sensing of proteins, based on polypeptide-functionalized gold nanoparticles. Recognition is accomplished using a polypeptide sensor scaffold designed to specifically bind to the model analyte, human carbonic anhydrase II (HCAII). The extent of particle aggregation, induced by the Zn2+-triggered dimerization and folding of a second polypeptide also present on the surface of the gold nanoparticle, gives a readily detectable colorimetric shift that is dependent on the concentration of the target protein. In the absence of HCAII, particle aggregation results in a major redshift of the plasmon peak, whereas analyte binding prevented the formation of dense aggregates, significantly reducing the magnitude of the redshift. The versatility of the technique is demonstrated using a second model system based on the recognition of a peptide sequence from the tobacco mosaic virus coat protein (TMVP) by a recombinant antibody fragment (Fab57P). Concentrations down to approximate to 10 nM and approximate to 25 nM are detected for HCAII and Fab57P, respectively. This strategy is proposed as a generic platform for robust and specific protein analysis that can be further developed to monitor a wide range of target proteins.
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Selegård, RobertLinköpings universitet,Molekylär fysik,Tekniska fakulteten(Swepub:liu)robse34
(author)
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Baltzer, LarsUppsala universitet,Institutionen för biokemi och organisk kemi,Division of Organic Chemistry, Department of Biochemistry and Organic Chemistry, BMC, Box 576, Uppsala University, SE-751 23 Uppsala, Sweden(Swepub:uu)labal087
(author)
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Enander, KarinLinköpings universitet,Molekylär fysik,Tekniska fakulteten(Swepub:liu)karen14
(author)
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Liedberg, BoLinköpings universitet,Molekylär fysik,Tekniska fakulteten(Swepub:liu)boli31
(author)
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Linköpings universitetMolekylär fysik
(creator_code:org_t)
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In:Small: Wiley5:21, s. 2445-24521613-68101613-6829
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