Direct spectroelectrochemistry of peroxidases immobilised on mesoporous metal oxide electrodes : Towards reagentless hydrogen peroxide sensing

• In this paper, we employ two peroxidases (horseradish peroxidase, HRP and cytochrome c peroxidase, CcP) to demonstrate their ability to retain their redox and biological functions after their immobilisation on mesoporous TiO2 and SnO2 electrodes. We will also demonstrate the use of HRP immobilised on the metal oxide electrodes for the development of reagentless optical and electrochemical biosensors for the detection of hydrogen peroxide (H2O2) with low detection limit of 0.04 and 1 mu M, respectively. Crown Copyright (C) 2009 Published by Elsevier B.V. All rights reserved.

Subject headings

NATURVETENSKAP  -- Kemi (hsv//swe)

NATURAL SCIENCES  -- Chemical Sciences (hsv//eng)

Keyword

Horseradish peroxidase

Cytochrome c peroxidase

Hydrogen peroxide

Biosensor

Titania

SnO2 electrodes

Chemistry

Kemi

Publication and Content Type

ref (subject category)

art (subject category)