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[C-11]PIB-amyloid binding and levels of A beta 40 and A beta 42 in postmortem brain tissue from Alzheimer patients
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- Svedberg, Marie M. (author)
- Uppsala universitet,Institutionen för biokemi och organisk kemi
- Hall, Håkan (author)
- Hellström-Lindahl, Ewa (author)
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- Estrada, Sergio (author)
- Guan, ZhiZhong (author)
- Nordberg, Agneta (author)
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- (creator_code:org_t)
- Elsevier BV, 2009
- 2009
- English.
- In: Neurochemistry International. - : Elsevier BV. - 0197-0186 .- 1872-9754. ; 54:5-6, s. 347-357
- Journal article (peer-reviewed)
Abstract
Subject headings
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- beta-Amyloid (A beta) deposits are one of the major histopathological hallmarks of Alzheimer's disease (AD). The amyloid-imaging positron emission tomography (PET) tracer [C-11]PIB (N-methyl[C-11]2-(4'-methylaminophenyl)-6-hydroxy-benzothiazole) is used in the assessment of A beta deposits in the human brain. [C-11]PIB-amyloid interaction and insoluble A beta 40 and A beta 42 peptide levels in the brain were quantified in postmortem tissue from nine AD patients and nine age-matched control subjects in the temporal, frontal and parietal cortices and the cerebellum. Autoradiographical studies showed significantly higher densities of specific [C-11]PIB-amyloid binding in gray matter in the temporal and parietal cortex (62 fmol/mg tissue) in AD patients as compared to control subjects, whereas the density was somewhat lower in the frontal cortex (56 fmol/mg tissue). No specific binding could be detected in the AD cerebellum or in the tissues from the control subjects (<= 5 fmol/mg tissue). Insoluble A beta 40 and total A beta levels (i.e. sum of A beta 40 and A beta 42) were significantly higher in patients than in controls in all measured cortical regions as determined using ELISA, which was confirmed using immunohistochemistry. The present findings show a more regional selective distribution of [C-11]PIB amyloid binding than previously reported. Moreover, it is suggested that some of the [C-11]PIB binding and insoluble A beta seen in control subjects may be amyloid in the blood vessels. (C) 2009 Elsevier Ltd. All rights reserved.
Subject headings
- NATURVETENSKAP -- Kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences (hsv//eng)
Keyword
- Alzheimer's disease
- Autoradiography
- beta-Amyloid
- [C-11]PIB
- Immunohistochemistry
- Radioligand assay
- Chemistry
- Kemi
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- ref (subject category)
- art (subject category)
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