SwePub
Sök i LIBRIS databas

  Extended search

onr:"swepub:oai:DiVA.org:uu-129274"
 

Search: onr:"swepub:oai:DiVA.org:uu-129274" > Helicobacter pylori...

  • 1 of 1
  • Previous record
  • Next record
  •    To hitlist
  • Sharma, Atul (author)

Helicobacter pylori single-stranded DNA binding protein - functional characterization and modulation of H-pylori DnaB helicase activity

  • Article/chapterEnglish2009

Publisher, publication year, extent ...

  • 2008-12-12
  • Wiley,2009
  • printrdacarrier

Numbers

  • LIBRIS-ID:oai:DiVA.org:uu-129274
  • https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-129274URI
  • https://doi.org/10.1111/j.1742-4658.2008.06799.xDOI

Supplementary language notes

  • Language:English
  • Summary in:English

Part of subdatabase

Classification

  • Subject category:ref swepub-contenttype
  • Subject category:art swepub-publicationtype

Notes

  • Helicobacter pylori, an important bacterial pathogen, causes gastric ulcer and gastric adenocarcinoma in humans. The fundamentals of basic biology such as DNA replication are poorly understood in this pathogen. In the present study, we report the cloning and functional characterization of the single-stranded DNA (ssDNA) binding protein from H. pylori. The N-terminal DNA binding domain shows significant homology with E. coli single-stranded DNA binding protein (SSB), whereas the C-terminal domain shows less homology. The overall DNA-binding activity and tetramerization properties, however, remain unaffected. In in vitro experiments with purified proteins, H. pylori (Hp) SSB bound specifically to ssDNA and modulated the enzymatic ATPase and helicase activity of HpDnaB helicase. HpSSB and HpDnaB proteins were co-localized in sharp, distinct foci in exponentially growing H. pylori cells, whereas both were spread over large areas in its dormant coccoid form, suggesting the absence of active replication forks in the latter. These results confirm the multiple roles of SSB during DNA replication and provide evidence for altered replicative metabolism in the spiral and coccoid forms that may be central to the bacterial physiology and pathogenesis.

Subject headings and genre

Added entries (persons, corporate bodies, meetings, titles ...)

  • Nitharwal, G (author)
  • Singh, BhupenderUppsala universitet,Institutionen för cell- och molekylärbiologi(Swepub:uu)bhsin121 (author)
  • Dar, Ashraf (author)
  • Dasgupta, SantanuUppsala universitet,Institutionen för cell- och molekylärbiologi (author)
  • Dhar, Suman K. (author)
  • Uppsala universitetInstitutionen för cell- och molekylärbiologi (creator_code:org_t)

Related titles

  • In:The FEBS Journal: Wiley276:2, s. 519-5311742-464X1742-4658

Internet link

Find in a library

To the university's database

  • 1 of 1
  • Previous record
  • Next record
  •    To hitlist

Find more in SwePub

By the author/editor
Sharma, Atul
Nitharwal, G
Singh, Bhupender
Dar, Ashraf
Dasgupta, Santan ...
Dhar, Suman K.
About the subject
NATURAL SCIENCES
NATURAL SCIENCES
and Biological Scien ...
Articles in the publication
The FEBS Journal
By the university
Uppsala University

Search outside SwePub

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

 
pil uppåt Close

Copy and save the link in order to return to this view