onr:"swepub:oai:DiVA.org:uu-129532"
Search: onr:"swepub:oai:DiVA.org:uu-129532" > Integrative epigeno...
- 1 of 1
- Previous record
- Next record
- To hitlist
Integrative epigenomic and genomic analysis of malignant pheochromocytoma
-
- Sandgren, Johanna (author)
- Uppsala universitet,Institutionen för kirurgiska vetenskaper,Institutionen för genetik och patologi
-
- Andersson, Robin (author)
- Uppsala universitet,Centrum för bioinformatik
-
- Rada-Iglesias, Alvaro (author)
- Uppsala universitet,Centrum för bioinformatik
- show more...
- show less...
- (creator_code:org_t)
- Springer Science and Business Media LLC, 2010
- 2010
- English.
- In: Experimental and Molecular Medicine. - : Springer Science and Business Media LLC. - 1226-3613 .- 2092-6413. ; 42:7, s. 484-502
- Journal article (peer-reviewed)
Abstract
Subject headings
Close
- Epigenomic and genomic changes affect gene expression and contribute to tumor development. The histone modifications trimethylated histone H3 lysine 4 (H3K4me3) and lysine 27 (H3K27me3) are epigenetic regulators associated to active and silenced genes, respectively and alterations of these modifications have been observed in cancer. Furthermore, genomic aberrations such as DNA copy number changes are common events in tumors. Pheochromocytoma is a rare endocrine tumor of the adrenal gland that mostly occurs sporadic with unknown epigenetic/genetic cause. The majority of cases are benign. Here we aimed to combine the genome-wide profiling of H3K4me3 and H3K27me3, obtained by the ChIP-chip methodology, and DNA copy number data with global gene expression examination in a malignant pheochromocytoma sample. The integrated analysis of the tumor expression levels, in relation to normal adrenal medulla, indicated that either histone modifications or chromosomal alterations, or both, have great impact on the expression of a substantial fraction of the genes in the investigated sample. Candidate tumor suppressor genes identified with decreased expression, a H3K27me3 mark and/or in regions of deletion were for instance TGIF1, DSC3, TNFRSF10B, RASSF2, HOXA9, PTPRE and CDH11. More genes were found with increased expression, a H3K4me3 mark, and/or in regions of gain. Potential oncogenes detected among those were GNAS, INSM1, DOK5, ETV1, RET, NTRK1, IGF2, and the H3K27 trimethylase gene EZH2. Our approach to associate histone methylations and DNA copy number changes to gene expression revealed apparent impact on global gene transcription, and enabled the identification of candidate tumor genes for further exploration.
Keyword
- histone code
- DNA copy number changes
- gene expression
- oncogenes
- pheochromocytoma
- tumor suppressor genes
- MEDICINE
- MEDICIN
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
- Experimental and Molecular Medicine (Search for host publication in LIBRIS)
To the university's database
- 1 of 1
- Previous record
- Next record
- To hitlist
Find more in SwePub
- By the author/editor
- Sandgren, Johann ...
- Andersson, Robin
- Rada-Iglesias, A ...
- Enroth, Stefan
- Åkerström, Göran
- Dumanski, Jan P.
- show more...
- Komorowski, Jan
- Westin, Gunnar
- Wadelius, Claes
- show less...
- Articles in the publication
- Experimental and ...
- By the university
- Uppsala University
Search outside SwePub
- Extend your search to:
- Google Book Search
- Google Scholar
Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.
- Copyright © LIBRIS - National Library Systems
- LIBRIS.kb.se
