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- Unoson, CeciliaUppsala universitet,Institutionen för cell- och molekylärbiologi (author)
Small RNA-mediated Regulation of Gene Expression in Escherichia coli
- BookEnglish2010
Publisher, publication year, extent ...
- Uppsala :Acta Universitatis Upsaliensis,2010
- 76 s.
- electronicrdacarrier
Numbers
- LIBRIS-ID:oai:DiVA.org:uu-130885
- ISBN:9789155478919
- https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-130885URI
Supplementary language notes
- Language:English
- Summary in:English
Part of subdatabase
- SwePubSwePub
Classification
Series
- Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology,1651-6214 ;767
Notes
- Non-coding RNAs are highly abundant regulators of gene expression in all kingdoms of life that often play important roles in vital cellular functions. In bacteria, small regulatory RNAs (sRNAs) usually act post-transcriptionally by regulating mRNAs through base pairing within ribosome binding sites (RBS), thereby inhibiting translation initiation. tisB encodes a toxin, TisB, whose synthesis is controlled by the sRNA IstR-1. Intriguingly, IstR-1 base pairs far upstream of the RBS but nevertheless inhibits translation initiation. The tisB mRNA is unusual in that ribosomes cannot access the RBS directly, but instead need an unstructured upstream region. This is precisely where IstR-1 exerts its inhibitory effect. We propose this region to serve as a ribosome loading site (standby site) which permits ribosomes to overcome the obstacle of inhibitory RBS-containing structures. Sequence-independent ribosome binding to the standby site allows for efficient relocation to the RBS structure when it is transiently open. Thus, standby sites are translation enhancer elements. I also characterized TisB-mediated toxicity. The hydrophobic protein TisB is targeted to the inner membrane and causes damage. This decreases the intracellular ATP concentration and entails decreased replication, transcription and translation rates. It is likely that this toxin is involved in multidrug tolerance under certain conditions. We identified the sRNA MicF as a negative regulator of lrp expression. Lrp is a global transcription factor that controls genes involved in amino acid metabolism and transport of small molecules. Interestingly, Lrp also downregulates MicF. Thus, this study established that the mutual downregulation of MicF/Lrp creates a positive feedback loop which gives a switch-like behavior important for fast adaptations.
Subject headings and genre
- NATURVETENSKAP Biologi Mikrobiologi hsv//swe
- NATURAL SCIENCES Biological Sciences Microbiology hsv//eng
- NATURVETENSKAP Biologi Biokemi och molekylärbiologi hsv//swe
- NATURAL SCIENCES Biological Sciences Biochemistry and Molecular Biology hsv//eng
- Small RNA
- non-coding RNA
- antisense
- translational control
- ribosome standby
- toxin-antitoxin
- IstR
- TisB
- tisAB
- MicF
- Lrp
- Microbiology
- Mikrobiologi
- Cell and molecular biology
- Cell- och molekylärbiologi
- Biologi med inriktning mot mikrobiologi
- Biology with specialization in Microbiology
Added entries (persons, corporate bodies, meetings, titles ...)
- Wagner, E. Gerhart. H,ProfessorUppsala universitet,Institutionen för cell- och molekylärbiologi (thesis advisor)
- Schroeder, Renée,ProfessorMFPL, University of Vienna (opponent)
- Uppsala universitetInstitutionen för cell- och molekylärbiologi (creator_code:org_t)
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