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Arachidonic acid is...
Arachidonic acid is a physiological activator of the ryanodine receptor in pancreatic beta-cells.
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Woolcott, Orison O (author)
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Gustafsson, Amanda J (author)
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- Dzabic, Mensur (author)
- Karolinska Institutet
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Pierro, Cristina (author)
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Tedeschi, Patrizia (author)
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- Sandgren, Johanna (author)
- Karolinska Institutet
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Bari, M Rizuanul (author)
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Nguyen, K Hoa (author)
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Bianchi, Marta (author)
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- Rakonjac, Marija (author)
- Karolinska Institutet
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- Rådmark, Olof (author)
- Karolinska Institutet
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- Ostenson, Claes-Göran (author)
- Karolinska Institutet
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- Islam, Md Shahidul (author)
- Karolinska Institutet
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(creator_code:org_t)
- Elsevier BV, 2006
- 2006
- English.
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In: Cell Calcium. - : Elsevier BV. - 0143-4160 .- 1532-1991. ; 39:6, s. 529-37
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
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- Pancreatic beta-cells have ryanodine receptors but little is known about their physiological regulation. Previous studies have shown that arachidonic acid releases Ca(2+) from intracellular stores in beta-cells but the identity of the channels involved in the Ca(2+) release has not been elucidated. We studied the mechanism by which arachidonic acid induces Ca(2+) concentration changes in pancreatic beta-cells. Cytosolic free Ca(2+) concentration was measured in fura-2-loaded INS-1E cells and in primary beta-cells from Wistar rats. The increase of cytosolic Ca(2+) concentration induced by arachidonic acid (150microM) was due to both Ca(2+) release from intracellular stores and influx of Ca(2+) from extracellular medium. 5,8,11,14-Eicosatetraynoic acid, a non-metabolizable analogue of arachidonic acid, mimicked the effect of arachidonic acid, indicating that arachidonic acid itself mediated Ca(2+) increase. The Ca(2+) release induced by arachidonic acid was from the endoplasmic reticulum since it was blocked by thapsigargin. 2-Aminoethyl diphenylborinate (50microM), which is known to inhibit 1,4,5-inositol-triphosphate-receptors, did not block Ca(2+) release by arachidonic acid. However, ryanodine (100microM), a blocker of ryanodine receptors, abolished the effect of arachidonic acid on Ca(2+) release in both types of cells. These observations indicate that arachidonic acid is a physiological activator of ryanodine receptors in beta-cells.
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- By the author/editor
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Woolcott, Orison ...
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Gustafsson, Aman ...
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Dzabic, Mensur
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Pierro, Cristina
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Tedeschi, Patriz ...
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Sandgren, Johann ...
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show more...
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Bari, M Rizuanul
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Nguyen, K Hoa
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Bianchi, Marta
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Rakonjac, Marija
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Rådmark, Olof
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Ostenson, Claes- ...
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Islam, Md Shahid ...
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show less...
- Articles in the publication
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Cell Calcium
- By the university
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Uppsala University
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Karolinska Institutet