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  • Woolcott, Orison O (author)

Arachidonic acid is a physiological activator of the ryanodine receptor in pancreatic beta-cells.

  • Article/chapterEnglish2006

Publisher, publication year, extent ...

  • Elsevier BV,2006
  • printrdacarrier

Numbers

  • LIBRIS-ID:oai:DiVA.org:uu-132138
  • https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-132138URI
  • https://doi.org/10.1016/j.ceca.2006.02.003DOI
  • http://kipublications.ki.se/Default.aspx?queryparsed=id:1950415URI

Supplementary language notes

  • Language:English
  • Summary in:English

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  • Subject category:ref swepub-contenttype
  • Subject category:art swepub-publicationtype

Notes

  • Pancreatic beta-cells have ryanodine receptors but little is known about their physiological regulation. Previous studies have shown that arachidonic acid releases Ca(2+) from intracellular stores in beta-cells but the identity of the channels involved in the Ca(2+) release has not been elucidated. We studied the mechanism by which arachidonic acid induces Ca(2+) concentration changes in pancreatic beta-cells. Cytosolic free Ca(2+) concentration was measured in fura-2-loaded INS-1E cells and in primary beta-cells from Wistar rats. The increase of cytosolic Ca(2+) concentration induced by arachidonic acid (150microM) was due to both Ca(2+) release from intracellular stores and influx of Ca(2+) from extracellular medium. 5,8,11,14-Eicosatetraynoic acid, a non-metabolizable analogue of arachidonic acid, mimicked the effect of arachidonic acid, indicating that arachidonic acid itself mediated Ca(2+) increase. The Ca(2+) release induced by arachidonic acid was from the endoplasmic reticulum since it was blocked by thapsigargin. 2-Aminoethyl diphenylborinate (50microM), which is known to inhibit 1,4,5-inositol-triphosphate-receptors, did not block Ca(2+) release by arachidonic acid. However, ryanodine (100microM), a blocker of ryanodine receptors, abolished the effect of arachidonic acid on Ca(2+) release in both types of cells. These observations indicate that arachidonic acid is a physiological activator of ryanodine receptors in beta-cells.

Added entries (persons, corporate bodies, meetings, titles ...)

  • Gustafsson, Amanda J (author)
  • Dzabic, MensurKarolinska Institutet (author)
  • Pierro, Cristina (author)
  • Tedeschi, Patrizia (author)
  • Sandgren, JohannaKarolinska Institutet (author)
  • Bari, M Rizuanul (author)
  • Nguyen, K Hoa (author)
  • Bianchi, Marta (author)
  • Rakonjac, MarijaKarolinska Institutet (author)
  • Rådmark, OlofKarolinska Institutet (author)
  • Ostenson, Claes-GöranKarolinska Institutet (author)
  • Islam, Md ShahidulKarolinska Institutet (author)
  • Karolinska Institutet (creator_code:org_t)

Related titles

  • In:Cell Calcium: Elsevier BV39:6, s. 529-370143-41601532-1991

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