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Arachidonic acid is...
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Woolcott, Orison O
(author)
Arachidonic acid is a physiological activator of the ryanodine receptor in pancreatic beta-cells.
- Article/chapterEnglish2006
Publisher, publication year, extent ...
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Elsevier BV,2006
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Numbers
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LIBRIS-ID:oai:DiVA.org:uu-132138
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https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-132138URI
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https://doi.org/10.1016/j.ceca.2006.02.003DOI
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http://kipublications.ki.se/Default.aspx?queryparsed=id:1950415URI
Supplementary language notes
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Language:English
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Summary in:English
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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Pancreatic beta-cells have ryanodine receptors but little is known about their physiological regulation. Previous studies have shown that arachidonic acid releases Ca(2+) from intracellular stores in beta-cells but the identity of the channels involved in the Ca(2+) release has not been elucidated. We studied the mechanism by which arachidonic acid induces Ca(2+) concentration changes in pancreatic beta-cells. Cytosolic free Ca(2+) concentration was measured in fura-2-loaded INS-1E cells and in primary beta-cells from Wistar rats. The increase of cytosolic Ca(2+) concentration induced by arachidonic acid (150microM) was due to both Ca(2+) release from intracellular stores and influx of Ca(2+) from extracellular medium. 5,8,11,14-Eicosatetraynoic acid, a non-metabolizable analogue of arachidonic acid, mimicked the effect of arachidonic acid, indicating that arachidonic acid itself mediated Ca(2+) increase. The Ca(2+) release induced by arachidonic acid was from the endoplasmic reticulum since it was blocked by thapsigargin. 2-Aminoethyl diphenylborinate (50microM), which is known to inhibit 1,4,5-inositol-triphosphate-receptors, did not block Ca(2+) release by arachidonic acid. However, ryanodine (100microM), a blocker of ryanodine receptors, abolished the effect of arachidonic acid on Ca(2+) release in both types of cells. These observations indicate that arachidonic acid is a physiological activator of ryanodine receptors in beta-cells.
Added entries (persons, corporate bodies, meetings, titles ...)
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Gustafsson, Amanda J
(author)
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Dzabic, MensurKarolinska Institutet
(author)
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Pierro, Cristina
(author)
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Tedeschi, Patrizia
(author)
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Sandgren, JohannaKarolinska Institutet
(author)
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Bari, M Rizuanul
(author)
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Nguyen, K Hoa
(author)
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Bianchi, Marta
(author)
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Rakonjac, MarijaKarolinska Institutet
(author)
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Rådmark, OlofKarolinska Institutet
(author)
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Ostenson, Claes-GöranKarolinska Institutet
(author)
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Islam, Md ShahidulKarolinska Institutet
(author)
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Karolinska Institutet
(creator_code:org_t)
Related titles
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In:Cell Calcium: Elsevier BV39:6, s. 529-370143-41601532-1991
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Dzabic, Mensur
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Pierro, Cristina
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Tedeschi, Patriz ...
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Bari, M Rizuanul
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Nguyen, K Hoa
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Cell Calcium
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Uppsala University
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Karolinska Institutet