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H-NS-mediated repre...
H-NS-mediated repression of CRISPR-based immunity in Escherichia coli K12 can be relieved by the transcription activator LeuO
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Westra, Edze R (author)
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Pul, Ümit (author)
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- Heidrich, Nadja (author)
- Uppsala universitet,Institutionen för cell- och molekylärbiologi
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Jore, Matthijs M (author)
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- Lundgren, Magnus (author)
- Uppsala universitet,Mikrobiologi
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Stratmann, Thomas (author)
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Wurm, Reinhild (author)
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- Raine, Amanda (author)
- Uppsala universitet,Institutionen för cell- och molekylärbiologi
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Mescher, Melina (author)
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van Heereveld, Luc (author)
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Mastop, Marieke (author)
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- Wagner, Gerhart E. H (author)
- Uppsala universitet,Institutionen för cell- och molekylärbiologi
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Schnetz, Karin (author)
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van der Oost, John (author)
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Wagner, Rolf (author)
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Brouns, Stan J. J. (author)
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(creator_code:org_t)
- 2010-09-14
- 2010
- English.
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In: Molecular Microbiology. - : Wiley. - 0950-382X .- 1365-2958. ; 77:6, s. 1380-1393
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
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- The recently discovered prokaryotic CRISPR/Cas defence system provides immunity against viral infections and plasmid conjugation. It has been demonstrated that in Escherichia coli transcription of the Cascade genes (casABCDE) and to some extent the CRISPR array is repressed by heat-stable nucleoid-structuring (H-NS) protein, a global transcriptional repressor. Here we elaborate on the control of the E. coli CRISPR/Cas system, and study the effect on CRISPR-based anti-viral immunity. Transformation of wild-type E. coli K12 with CRISPR spacers that are complementary to phage Lambda does not lead to detectable protection against Lambda infection. However, when an H-NS mutant of E. coli K12 is transformed with the same anti-Lambda CRISPR, this does result in reduced sensitivity to phage infection. In addition, it is demonstrated that LeuO, a LysR-type transcription factor, binds to two sites flanking the casA promoter and the H-NS nucleation site, resulting in derepression of casABCDE12 transcription. Overexpression of LeuO in E. coli K12 containing an anti-Lambda CRISPR leads to an enhanced protection against phage infection. This study demonstrates that in E. coli H-NS and LeuO are antagonistic regulators of CRISPR-based immunity.
Subject headings
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
Keyword
- Biology
- Biologi
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Westra, Edze R
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Pul, Ümit
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Heidrich, Nadja
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Jore, Matthijs M
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Lundgren, Magnus
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Stratmann, Thoma ...
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show more...
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Wurm, Reinhild
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Raine, Amanda
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Mescher, Melina
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van Heereveld, L ...
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Mastop, Marieke
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Wagner, Gerhart ...
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Schnetz, Karin
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van der Oost, Jo ...
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Wagner, Rolf
-
Brouns, Stan J. ...
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show less...
- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
- Articles in the publication
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Molecular Microb ...
- By the university
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Uppsala University