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Nuclear deadenylati...
Nuclear deadenylation/polyadenylation factors regulate 3 ' processing in response to DNA damage
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Cevher, Murat A. (author)
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Zhang, Xiaokan (author)
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Fernandez, Sully (author)
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Kim, Sergey (author)
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Baquero, Jorge (author)
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- Nilsson, Per (author)
- Karolinska Institutet,Uppsala universitet,Institutionen för cell- och molekylärbiologi
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Lee, Sean (author)
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- Virtanen, Anders (author)
- Uppsala universitet,Institutionen för cell- och molekylärbiologi
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Kleiman, Frida E. (author)
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(creator_code:org_t)
- 2010-04-08
- 2010
- English.
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In: EMBO Journal. - : Wiley. - 0261-4189 .- 1460-2075. ; 29:10, s. 1674-1687
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https://europepmc.or...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
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- We previously showed that mRNA 3' end cleavage reaction in cell extracts is strongly but transiently inhibited under DNA-damaging conditions. The cleavage stimulation factor-50 (CstF-50) has a role in this response, providing a link between transcription-coupled RNA processing and DNA repair. In this study, we show that CstF-50 interacts with nuclear poly(A)-specific ribonuclease (PARN) using in vitro and in extracts of UV-exposed cells. The CstF-50/PARN complex formation has a role in the inhibition of 3' cleavage and activation of deadenylation upon DNA damage. Extending these results, we found that the tumour suppressor BARD1, which is involved in the UV-induced inhibition of 3' cleavage, strongly activates deadenylation by PARN in the presence of CstF-50, and that CstF-50/BARD1 can revert the cap-binding protein-80 (CBP80)mediated inhibition of PARN activity. We also provide evidence that PARN along with the CstF/BARD1 complex participates in the regulation of endogenous transcripts under DNA-damaging conditions. We speculate that the interplay between polyadenylation, deadenylation and tumour-suppressor factors might prevent the expression of prematurely terminated messengers, contributing to control of gene expression under different cellular conditions.
Subject headings
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
Keyword
- 3 ' RNA processing
- deadenylation
- DNA damage
- polyadenylation
- Biology
- Biologi
Publication and Content Type
- ref (subject category)
- art (subject category)
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