Search: onr:"swepub:oai:DiVA.org:uu-136907" >
Tissue localization...
Tissue localization and the establishment of a sensitive immunoassay of the newly discovered human phospholipase B-precursor (PLB-P)
-
- Xu, Shengyuan (author)
- Uppsala universitet,Klinisk kemi
-
- Cai, Linjun (author)
- Uppsala universitet,Klinisk kemi
-
- Zhao, Linshu (author)
- Uppsala universitet,Klinisk kemi
-
show more...
-
- Douhan-Håkansson, Lena (author)
- Uppsala universitet,Klinisk kemi
-
Kristjansson, Gudjon (author)
-
- Pauksen, Karlis (author)
- Uppsala universitet,Infektionssjukdomar
-
- Venge, Per (author)
- Uppsala universitet,Klinisk kemi
-
show less...
-
(creator_code:org_t)
- Elsevier BV, 2010
- 2010
- English.
-
In: JIM - Journal of Immunological Methods. - : Elsevier BV. - 0022-1759 .- 1872-7905. ; 353:1-2, s. 71-77
- Related links:
-
https://urn.kb.se/re...
-
show more...
-
https://doi.org/10.1...
-
show less...
Abstract
Subject headings
Close
- Human phospholipase B-precursor (PLB-P) is a newly identified and purified protein from human neutrophils. The precise function of PLB-P in vivo is not yet known. Its existence in neutrophils and the enzymatic activity against phospholipids imply a role in the defence against invading microorganisms and in the generation of lipid mediators of inflammation. We describe here the generation of specific antibodies against PLB-P, the tissue localizations of PLB-P and the establishment of an accurate, specific, and reproducible radioimmunoassay (RIA). A survey of normal and malignant tissues showed strong immunostaining of PLB-P in neuronal and myeloid cells and in adrenal glands. Elevated levels were found in sera of patients with influenza A infection i.e. > 1 mu g/L and in gut fluids of patients with inflammatory bowel disease i.e. > 20 mu g/L. The levels correlated to markets of neutrophil activation, suggesting a neutrophil origin of PLB-P in these conditions. The antibodies and the assay will be useful in the future basic and clinical investigations of PLB-P.
Keyword
- Phospholipase B
- Radioimmunoassay
- Neutrophils
- Infection
- Inflammation
- MEDICINE
- MEDICIN
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
To the university's database