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Interlocking disulf...
Interlocking disulfides in circular proteins : toward efficient oxidative folding of cyclotides.
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- Aboye, Teshome Leta (author)
- Uppsala universitet,Institutionen för läkemedelskemi,Avdelningen för farmakognosi
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- Clark, Richard J. (author)
- Uppsala universitet,Institutionen för läkemedelskemi,Avdelningen för farmakognosi
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- Burman, Robert (author)
- Uppsala universitet,Institutionen för läkemedelskemi,Avdelningen för farmakognosi
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- Roig, Marta Bajona (author)
- Uppsala universitet,Institutionen för läkemedelskemi,Avdelningen för farmakognosi
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- Craik, David J. (author)
- University of Queensland, Institute for Molecular Bioscience
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- Göransson, Ulf (author)
- Uppsala universitet,Institutionen för läkemedelskemi,Avdelningen för farmakognosi
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(creator_code:org_t)
- Mary Ann Liebert Inc, 2011
- 2011
- English.
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In: Antioxidants and Redox Signaling. - : Mary Ann Liebert Inc. - 1523-0864 .- 1557-7716. ; 14:1, s. 77-86
- Related links:
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https://espace.libra...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
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- Cyclotides are ultrastable plant proteins characterized by the presence of a cyclic amide backbone and three disulfide bonds that form a cystine knot. Because of their extreme stability, there has been significant interest in developing these molecules as a drug design scaffold. For this potential to be realized, efficient methods for the synthesis and oxidative folding of cyclotides need to be developed, yet we currently have only a basic understanding of the folding mechanism and the factors influencing this process. In this study, we determine the major factors influencing oxidative folding of the different subfamilies of cyclotides. The folding of all the cyclotides examined was heavily influenced by the concentration of redox reagents, with the folding rate and final yield of the native isomer greatly enhanced by high concentrations of oxidized glutathione. Addition of hydrophobic solvents to the buffer also enhanced the folding rates and appeared to alter the folding pathway. Significant deamidation and isoaspartate formation were seen when oxidation conditions were conducive to slow folding. The identification of factors that influence the folding and degradation pathways of cyclotides will facilitate the development of folding screens and optimized conditions for producing cyclotides and grafted analogs as stable peptide-based therapeutics.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Farmaceutiska vetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Pharmaceutical Sciences (hsv//eng)
Keyword
- PHARMACY
- FARMACI
Publication and Content Type
- ref (subject category)
- art (subject category)
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