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Single skeletal mus...
Single skeletal muscle fiber elastic and contractile characteristics in young and older men
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- Ochala, Julien (author)
- Uppsala universitet,Institutionen för neurovetenskap
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Frontera, Walter R. (author)
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Dorer, David J. (author)
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Van Hoecke, Jacques (author)
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Krivickas, Lisa S. (author)
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(creator_code:org_t)
- 2007
- 2007
- English.
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In: The journals of gerontology. Series A, Biological sciences and medical sciences. - 1079-5006 .- 1758-535X. ; 62:4, s. 375-381
- Related links:
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https://urn.kb.se/re...
Abstract
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- The current investigation was designed to: (a) assess the impact of aging on elastic characteristics of single skeletal muscle fibers from young (N = 6) and older men (N = 6); and (b) correlate the potential changes, with the fiber contractile properties. Chemically skinned single muscle fibers (n = 235) from vastus lateralis muscle were maximally activated. Maximal force and cross-sectional area were measured, and specific force calculated. The slack test was used to measure maximal unloaded shortening velocity. A quick release of 0.15% of fiber length was applied to determine instantaneous stiffness. The myosin heavy chain isoform composition of each single fiber was determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Aging induces changes in both fiber elasticity (i.e., increased instantaneous stiffness) and contractility (i.e., reduced specific force and unloaded shortening velocity) in type I and IIa fibers. However, the changes in fiber stiffness may not directly influence contractile characteristics alterations.
Keyword
- MEDICINE
- MEDICIN
Publication and Content Type
- ref (subject category)
- art (subject category)
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