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Single mimivirus pa...
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Seibert, M. MarvinUppsala universitet,Molekylär biofysik
(author)
Single mimivirus particles intercepted and imaged with an X-ray laser
- Article/chapterEnglish2011
Publisher, publication year, extent ...
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2011-02-02
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Springer Science and Business Media LLC,2011
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printrdacarrier
Numbers
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LIBRIS-ID:oai:DiVA.org:uu-146069
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https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-146069URI
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https://doi.org/10.1038/nature09748DOI
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https://res.slu.se/id/publ/33573URI
Supplementary language notes
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Language:English
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Summary in:English
Part of subdatabase
Classification
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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X-ray lasers offer new capabilities in understanding the structure of biological systems, complex materials and matter under extreme conditions(1-4). Very short and extremely bright, coherent X-ray pulses can be used to outrun key damage processes and obtain a single diffraction pattern from a large macromolecule, a virus or a cell before the sample explodes and turns into plasma(1). The continuous diffraction pattern of non-crystalline objects permits oversampling and direct phase retrieval(2). Here we show that high-quality diffraction data can be obtained with a single X-ray pulse from a noncrystalline biological sample, a single mimivirus particle, which was injected into the pulsed beam of a hard-X-ray free-electron laser, the Linac Coherent Light Source(5). Calculations indicate that the energy deposited into the virus by the pulse heated the particle to over 100,000 K after the pulse had left the sample. The reconstructed exit wavefront (image) yielded 32-nm full-period resolution in a single exposure and showed no measurable damage. The reconstruction indicates inhomogeneous arrangement of dense material inside the virion. We expect that significantly higher resolutions will be achieved in such experiments with shorter and brighter photon pulses focused to a smaller area. The resolution in such experiments can be further extended for samples available in multiple identical copies.
Subject headings and genre
Added entries (persons, corporate bodies, meetings, titles ...)
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Ekeberg, TomasUppsala universitet,Molekylär biofysik(Swepub:uu)tomek611
(author)
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Maia, Filipe R. N. C.Uppsala universitet,Molekylär biofysik(Swepub:uu)cajca839
(author)
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Svenda, MartinUppsala universitet,Molekylär biofysik(Swepub:uu)msv31659
(author)
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Andreasson, JakobUppsala universitet,Molekylär biofysik(Swepub:uu)jakan998
(author)
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Joensson, OlofUppsala universitet,Molekylär biofysik
(author)
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Odic, DuskoUppsala universitet,Molekylär biofysik
(author)
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Iwan, BiancaUppsala universitet,Molekylär biofysik(Swepub:uu)biaiw419
(author)
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Rocker, AndreaUppsala universitet,Molekylär biofysik
(author)
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Westphal, DanielUppsala universitet,Molekylär biofysik(Swepub:uu)danwe345
(author)
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Hantke, MaxUppsala universitet,Molekylär biofysik(Swepub:uu)maxha207
(author)
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DePonte, Daniel P.
(author)
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Barty, Anton
(author)
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Schulz, Joachim
(author)
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Gumprecht, Lars
(author)
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Coppola, Nicola
(author)
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Aquila, Andrew
(author)
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Liang, Mengning
(author)
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White, Thomas A.
(author)
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Martin, Andrew
(author)
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Caleman, CarlUppsala universitet,Molekylär biofysik(Swepub:uu)cajca839
(author)
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Stern, Stephan
(author)
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Abergel, Chantal
(author)
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Seltzer, Virginie
(author)
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Claverie, Jean-Michel
(author)
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Bostedt, Christoph
(author)
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Bozek, John D.
(author)
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Boutet, Sebastien
(author)
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Miahnahri, A. Alan
(author)
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Messerschmidt, Marc
(author)
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Krzywinski, Jacek
(author)
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Williams, Garth
(author)
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Hodgson, Keith O.
(author)
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Bogan, Michael J.
(author)
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Hampton, Christina Y.
(author)
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Sierra, Raymond G.
(author)
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Starodub, Dmitri
(author)
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Andersson, IngerSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för molekylärbiologi,Department of Molecular Biology(Swepub:slu)47211
(author)
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Bajt, Sasa
(author)
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Barthelmess, Miriam
(author)
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Spence, John C. H.
(author)
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Fromme, Petra
(author)
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Weierstall, Uwe
(author)
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Kirian, Richard
(author)
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Hunter, Mark
(author)
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Doak, R. Bruce
(author)
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Marchesini, Stefano
(author)
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Hau-Riege, Stefan P.
(author)
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Frank, Matthias
(author)
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Shoeman, Robert L.
(author)
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Lomb, Lukas
(author)
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Epp, Sascha W.
(author)
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Hartmann, Robert
(author)
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Rolles, Daniel
(author)
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Rudenko, Artem
(author)
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Schmidt, Carlo
(author)
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Foucar, Lutz
(author)
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Kimmel, Nils
(author)
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Holl, Peter
(author)
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Rudek, Benedikt
(author)
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Erk, Benjamin
(author)
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Hoemke, Andre
(author)
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Reich, Christian
(author)
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Pietschner, Daniel
(author)
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Weidenspointner, Georg
(author)
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Strueder, Lothar
(author)
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Hauser, Guenter
(author)
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Gorke, Hubert
(author)
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Ullrich, Joachim
(author)
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Schlichting, Ilme
(author)
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Herrmann, Sven
(author)
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Schaller, Gerhard
(author)
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Schopper, Florian
(author)
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Soltau, Heike
(author)
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Kuehnel, Kai-Uwe
(author)
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Andritschke, Robert
(author)
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Schroeter, Claus-Dieter
(author)
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Krasniqi, Faton
(author)
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Bott, Mario
(author)
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Schorb, Sebastian
(author)
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Rupp, Daniela
(author)
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Adolph, Marcus
(author)
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Gorkhover, Tais
(author)
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Hirsemann, Helmut
(author)
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Potdevin, Guillaume
(author)
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Graafsma, Heinz
(author)
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Nilsson, Björn
(author)
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Chapman, Henry N.
(author)
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Hajdu, JanosUppsala universitet,Molekylär biofysik(Swepub:uu)janohajd
(author)
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Uppsala universitetMolekylär biofysik
(creator_code:org_t)
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Sveriges lantbruksuniversitet
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In:Nature: Springer Science and Business Media LLC470:7332, s. 78-810028-08361476-4687
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Seibert, M. Marv ...
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Ekeberg, Tomas
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Maia, Filipe R. ...
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Svenda, Martin
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Andreasson, Jako ...
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Joensson, Olof
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Odic, Dusko
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Iwan, Bianca
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Rocker, Andrea
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Westphal, Daniel
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Hantke, Max
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DePonte, Daniel ...
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Barty, Anton
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Schulz, Joachim
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Gumprecht, Lars
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Coppola, Nicola
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Aquila, Andrew
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Liang, Mengning
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White, Thomas A.
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Martin, Andrew
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Caleman, Carl
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Stern, Stephan
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Abergel, Chantal
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Seltzer, Virgini ...
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Claverie, Jean-M ...
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Bostedt, Christo ...
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Bozek, John D.
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Boutet, Sebastie ...
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Miahnahri, A. Al ...
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Messerschmidt, M ...
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Krzywinski, Jace ...
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Williams, Garth
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Hodgson, Keith O ...
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Bogan, Michael J ...
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Hampton, Christi ...
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Sierra, Raymond ...
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Starodub, Dmitri
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Andersson, Inger
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Bajt, Sasa
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Barthelmess, Mir ...
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Spence, John C. ...
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Fromme, Petra
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Weierstall, Uwe
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Kirian, Richard
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Hunter, Mark
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Doak, R. Bruce
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Marchesini, Stef ...
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Hau-Riege, Stefa ...
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Frank, Matthias
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Shoeman, Robert ...
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Lomb, Lukas
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Epp, Sascha W.
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Hartmann, Robert
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Rolles, Daniel
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Rudenko, Artem
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Schmidt, Carlo
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Foucar, Lutz
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Kimmel, Nils
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Holl, Peter
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Rudek, Benedikt
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Erk, Benjamin
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Hoemke, Andre
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Reich, Christian
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Pietschner, Dani ...
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Weidenspointner, ...
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Strueder, Lothar
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Hauser, Guenter
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Gorke, Hubert
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Ullrich, Joachim
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Schlichting, Ilm ...
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Herrmann, Sven
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Schaller, Gerhar ...
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Schopper, Floria ...
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Soltau, Heike
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Kuehnel, Kai-Uwe
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Andritschke, Rob ...
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Schroeter, Claus ...
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Krasniqi, Faton
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Bott, Mario
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Schorb, Sebastia ...
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Rupp, Daniela
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Adolph, Marcus
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Gorkhover, Tais
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Hirsemann, Helmu ...
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Potdevin, Guilla ...
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Graafsma, Heinz
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Nilsson, Björn
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Chapman, Henry N ...
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