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Structural Basis of...
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Dasgupta, Jhimli
(author)
Structural Basis of Oligosaccharide Receptor Recognition by Human Papillomavirus
- Article/chapterEnglish2011
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Numbers
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LIBRIS-ID:oai:DiVA.org:uu-148608
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https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-148608URI
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https://doi.org/10.1074/jbc.M110.160184DOI
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Language:English
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Summary in:English
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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High risk human papillomavirus types 16 (HPV16) and 18 (HPV18) can cause cervical cancer. Efficient infection by HPV16 and HPV18 pseudovirions requires interactions of particles with cell-surface receptor heparan sulfate oligosaccharide. To understand the virus-receptor interactions for HPV infection, we determined the crystal structures of HPV16 and HPV18 capsids bound to the oligosaccharide receptor fragment using oligomeric heparin. The HPV-heparin structures revealed multiple binding sites for the highly negatively charged oligosaccharide fragment on the capsid surface, which is different from previously reported virus-receptor interactions in which a single type of binding pocket is present for a particular receptor. We performed structure-guided mutagenesis to generate mutant viruses, and cell binding and infectivity assays demonstrated the functional role of viral residues involved in heparin binding. These results provide a basis for understanding virus-heparan sulfate receptor interactions critical for HPV infection and for the potential development of inhibitors against HPV infection.
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Bienkowska-Haba, Malgorzata
(author)
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Ortega, Marcos E.
(author)
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Patel, Hetalkumar D.
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Bodevin, SabrinaUppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi
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Spillmann, DorotheUppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi(Swepub:uu)dsp11476
(author)
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Bishop, Brooke
(author)
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Sapp, Martin
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Chen, Xiaojiang S.
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Uppsala universitetInstitutionen för medicinsk biokemi och mikrobiologi
(creator_code:org_t)
Related titles
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In:Journal of Biological Chemistry286:4, s. 2617-26240021-92581083-351X
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