PTAA and B10: new approaches to amyloid detection in tissue-evaluation of amyloid detection in tissue with a conjugated polyelectrolyte and a fibril-specific antibody fragment

PTAA and B10 : new approaches to amyloid detection in tissue-evaluation of amyloid detection in tissue with a conjugated polyelectrolyte and a fibril-specific antibody fragment

Kieninger, Barbara (author)

Gioeva, Zarina (author)

Krueger, Sandra (author)

Westermark, Gunilla T. (author): Uppsala universitet,Institutionen för medicinsk cellbiologi

Friedrich, Ralf P. (author)

Faendrich, Marcus (author)

Roecken, Christoph (author)

(creator_code:org_t)

2011-03-14
2011
English.
In: Amyloid. - : Informa UK Limited. - 1350-6129 .- 1744-2818. ; 18:2, s. 47-52

Related links:: https://urn.kb.se/re...; show more...; https://doi.org/10.3...; show less...

Abstract Subject headings

Methods: aEuro integral We compared the amyloid detection of PTAA and B10 to Congo red in 106 amyloid-containing tissue biopsies of diverse anatomical and precursor origin by evaluating the accordance in four grades (grade 0: no staining, grade 1: staining of < 33%% of the amyloid deposits, grade 2: 33--66%% and grade 3: aEuroS > 66%%). Results: aEuro integral PTAA showed grade 2--3 staining in 57 (54%%) cases, while B10 presented this accordance in only 25 (24%%) tissue biopsies. Grade 1 staining was found in 11 (10%%) samples with PTAA and in 62 (58%%) cases with B10. No staining at all (grade 0) occurred in 38 (36%%) biopsies when using PTAA and in 19 (18%%) cases when using B10. Conclusion: aEuro integral Although conformation-sensitive detection seemed promising, PTAA and B10 stain only a fraction of the examined amyloid samples when using routine surgical pathology settings. This study emphasises the necessity of having optimised pre-analytical protocols for recovery, storage and handling of samples if these novel amyloid ligands are to be used in routine diagnosis of amyloid.

By the author/editor: Kieninger, Barba ...; Gioeva, Zarina; Krueger, Sandra; Westermark, Guni ...; Friedrich, Ralf ...; Faendrich, Marcu ...; show more...; Roecken, Christo ...; show less...

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