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Gustavsson, Inger,1965-Uppsala universitet,Genomik,Cervical cancer group
(author)
Type-specific detection of high-risk human papillomavirus (HPV) in self-sampled cervicovaginal cells applied to FTA elute cartridge
- Article/chapterEnglish2011
Publisher, publication year, extent ...
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Elsevier BV,2011
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Numbers
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LIBRIS-ID:oai:DiVA.org:uu-156562
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https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-156562URI
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https://doi.org/10.1016/j.jcv.2011.05.006DOI
Supplementary language notes
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Language:English
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Summary in:English
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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BackgroundMost procedures for self-sampling of cervical cells are based on liquid-based media for transportation and storage. An alternative is to use a solid support, such as dry filter paper media.ObjectivesTo evaluate if self-sampling of cervicovaginal fluid using a cytobrush (Viba-brush; Rovers Medical Devices B.V., Oss, The Netherlands) and a solid support such as the Whatman Indicating FTA® Elute cartridge (GE Healthcare, United Kingdom) can be used for reliable typing of human papillomavirus (HPV), as compared to cervical samples obtained by a physician using a cytobrush and the indicating FTA® Elute Micro card and biopsy analysis.Study designA total of 50 women with a previous high-risk (HR) HPV positive test were invited to perform self-sampling using the Viba-brush and the FTA cartridge and thereafter a physician obtained a cervical sample using the cytobrush and a FTA card, together with a cervical biopsy for histology and HPV typing. Detection of HR-HPV types 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58 and 59 was performed using three multiplex real-time polymerase chain reaction (PCR) assays.ResultAll samples contained sufficient amounts of genomic DNA and the self-samples yielded on average 3.5 times more DNA than those obtained by the physician. All women that were positive for HR-HPV in the biopsy sample also typed positive both by self-sampling and physician-obtained sampling. For women with a histological diagnosis of cervical intraepithelial neoplasia grades 2–3 (CIN 2–3) all three HPV samples showed 100% concordance. A higher number of women were HPV positive by self-sampling than by physician-obtained sampling or by biopsy analysis.ConclusionThe Viba-brush and the FTA cartridge are suitable for self-sampling of vaginal cells and subsequent HR-HPV typing.
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Sanner, KarinUppsala universitet,Obstetrik & gynekologi(Swepub:uu)karisann
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Lindell, Monica
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Strand, AndersUppsala universitet,Dermatologi och venereologi(Swepub:uu)andestra
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Olovsson, MattsUppsala universitet,Obstetrik & gynekologi(Swepub:uu)mattolov
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Wikström, IngridUppsala universitet,Obstetrik & gynekologi(Swepub:uu)ingrwiks
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Wilander, ErikUppsala universitet,Institutionen för immunologi, genetik och patologi(Swepub:uu)erikwila
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Gyllensten, UlfUppsala universitet,Genomik(Swepub:uu)ulfgyll
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Uppsala universitetGenomik
(creator_code:org_t)
Related titles
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In:Journal of Clinical Virology: Elsevier BV51:4, s. 255-2581386-65321873-5967
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