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- Gustavsson, Inger,1965-Uppsala universitet,Genomik,Cervical cancer group (author)
Type-specific detection of high-risk human papillomavirus (HPV) in self-sampled cervicovaginal cells applied to FTA elute cartridge
- Article/chapterEnglish2011
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- Elsevier BV,2011
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- LIBRIS-ID:oai:DiVA.org:uu-156562
- https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-156562URI
- https://doi.org/10.1016/j.jcv.2011.05.006DOI
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- Language:English
- Summary in:English
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- BackgroundMost procedures for self-sampling of cervical cells are based on liquid-based media for transportation and storage. An alternative is to use a solid support, such as dry filter paper media.ObjectivesTo evaluate if self-sampling of cervicovaginal fluid using a cytobrush (Viba-brush; Rovers Medical Devices B.V., Oss, The Netherlands) and a solid support such as the Whatman Indicating FTA® Elute cartridge (GE Healthcare, United Kingdom) can be used for reliable typing of human papillomavirus (HPV), as compared to cervical samples obtained by a physician using a cytobrush and the indicating FTA® Elute Micro card and biopsy analysis.Study designA total of 50 women with a previous high-risk (HR) HPV positive test were invited to perform self-sampling using the Viba-brush and the FTA cartridge and thereafter a physician obtained a cervical sample using the cytobrush and a FTA card, together with a cervical biopsy for histology and HPV typing. Detection of HR-HPV types 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58 and 59 was performed using three multiplex real-time polymerase chain reaction (PCR) assays.ResultAll samples contained sufficient amounts of genomic DNA and the self-samples yielded on average 3.5 times more DNA than those obtained by the physician. All women that were positive for HR-HPV in the biopsy sample also typed positive both by self-sampling and physician-obtained sampling. For women with a histological diagnosis of cervical intraepithelial neoplasia grades 2–3 (CIN 2–3) all three HPV samples showed 100% concordance. A higher number of women were HPV positive by self-sampling than by physician-obtained sampling or by biopsy analysis.ConclusionThe Viba-brush and the FTA cartridge are suitable for self-sampling of vaginal cells and subsequent HR-HPV typing.
Subject headings and genre
- MEDICIN OCH HÄLSOVETENSKAP Medicinska och farmaceutiska grundvetenskaper Mikrobiologi inom det medicinska området hsv//swe
- MEDICAL AND HEALTH SCIENCES Basic Medicine Microbiology in the medical area hsv//eng
- Self-sampling
- FTA cartridge
- Cervical carcinoma
- Human papillomavirus (HPV)
- Real-time polymerase chain reaction (PCR)
- Clinical virology
- Klinisk virologi
Added entries (persons, corporate bodies, meetings, titles ...)
- Sanner, KarinUppsala universitet,Obstetrik & gynekologi(Swepub:uu)karisann (author)
- Lindell, Monica (author)
- Strand, AndersUppsala universitet,Dermatologi och venereologi(Swepub:uu)andestra (author)
- Olovsson, MattsUppsala universitet,Obstetrik & gynekologi(Swepub:uu)mattolov (author)
- Wikström, IngridUppsala universitet,Obstetrik & gynekologi(Swepub:uu)ingrwiks (author)
- Wilander, ErikUppsala universitet,Institutionen för immunologi, genetik och patologi(Swepub:uu)erikwila (author)
- Gyllensten, UlfUppsala universitet,Genomik(Swepub:uu)ulfgyll (author)
- Uppsala universitetGenomik (creator_code:org_t)
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- In:Journal of Clinical Virology: Elsevier BV51:4, s. 255-2581386-65321873-5967
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