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Simultaneous analys...
Simultaneous analysis of five antidepressant drugs using direct injection of biofluids in a capillary restricted-access media-liquid chromatography-tandem mass spectrometry system
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- Santos-Neto, Alvaro J. (author)
- Uppsala universitet,Analytisk kemi
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- Bergquist, Jonas (author)
- Uppsala universitet,Analytisk kemi
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Lancas, Fernando M. (author)
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- Sjöberg, Per J.R. (author)
- Uppsala universitet,Analytisk kemi
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(creator_code:org_t)
- Elsevier BV, 2008
- 2008
- English.
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In: Journal of Chromatography A. - : Elsevier BV. - 0021-9673 .- 1873-3778. ; 1189:1-2, s. 514-522
- Related links:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
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- Direct analysis, with minimal sample pretreatment, of antidepressant drugs, fluoxetine, imipramine, desipramine, amitriptyline, and nortriptyline in biofluids was developed with a total run time of 8 min. The setup consists of two HPLC pumps, injection valve, capillary RAM-ADS-C18 pre-column and a capillary analytical C 18 column connected by means of a six-port valve in backflush mode. Detection was performed with ESI-MS/MS and only 1 mu m of sample was injected. Validation was adequately carried out using FLU-d(5) as internal standard. Calibration curves were constructed under a linear range of 1-250 ng mL(-1) in plasma, being the limit of quantification (LOQ), determined as 1 ng mL(-1), for all the analytes. With the described approach it was possible to reach a quantified mass sensitivity of 0.3 pg for each analyte (equivalent to 1.1-1.3 fmol), translating to a lower sample consumption (in the order of 103 less sample than using conventional methods).
Subject headings
- NATURVETENSKAP -- Kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences (hsv//eng)
Keyword
- Column switching
- Restricted-access media (RAM)
- Capillary liquid chromatography
- Tandem mass spectrometry
- Antidepressant drugs
- Sample preparation automation
- Chemistry
- Kemi
Publication and Content Type
- ref (subject category)
- art (subject category)
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