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Analysis of short D...
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Suyama, Yoshihisa
(author)
Analysis of short DNA fragments from Holocene peatmoss samples
- Article/chapterEnglish2008
Publisher, publication year, extent ...
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2008-09-01
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SAGE Publications,2008
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printrdacarrier
Numbers
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LIBRIS-ID:oai:DiVA.org:uu-16260
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https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-16260URI
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https://doi.org/10.1177/0959683608093540DOI
Supplementary language notes
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Language:English
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Summary in:English
Part of subdatabase
Classification
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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This paper describes our recent attempt to isolate and analyse DNA from old plant remains of the common peatmoss Sphagnum fuscum retrieved from a peat core collected in the mire Fuglmyra, in central Norway. DNA was recoverable and usable from sub-fossilized (10–450 years old) plant remains of the peatmoss. A chloroplast (trnL) and two nuclear (ITS2 and RAPDf) regions were co-amplified from 80 samples of different ages. The RAPDf region was the only variable one with three different haplotypes found among five samples. Comparison of the ancient sequences with modern sequences found in the extant population occurring at the same site ascertained a genetic link between modern and fossil samples of this species. This retrieval of ancient DNA from sub-fossilized moss remains isolated from peat cores has important implications for the palaeoecology of peatmosses by allowing direct estimates of plant population dynamics in space and time.
Subject headings and genre
Added entries (persons, corporate bodies, meetings, titles ...)
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Gunnarsson, UrbanUppsala universitet,Ekologisk botanik(Swepub:uu)urbagunn
(author)
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Parducci, LauraUppsala universitet,Evolutionär funktionsgenomik(Swepub:uu)lapar168
(author)
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Uppsala universitetEkologisk botanik
(creator_code:org_t)
Related titles
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In:The Holocene: SAGE Publications18:6, s. 1003-10060959-68361477-0911
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