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Simultaneous genoty...
Simultaneous genotyping of all hemagglutinin and neuraminidase subtypes of avian influenza viruses by use of padlock probes
- Article/chapterEnglish2008
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Numbers
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LIBRIS-ID:oai:DiVA.org:uu-16412
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https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-16412URI
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https://doi.org/10.1128/JCM.02292-07DOI
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Language:English
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Summary in:English
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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A subtyping assay for both the hemagglutinin (HA) and neuraminidase (NA) surface antigens of the avian influenza virus (AIV) has been developed. The method uses padlock probe chemistry combined with a microarray output for detection. The outstanding feature of this assay is its capability to designate both the HA and the NA of an AIV sample from a single reaction mixture. A panel of 77 influenza virus strains was tested representing the entire assortment of the two antigens. One hundred percent (77/77) of the samples tested were identified as AIV, and 97% (75/77) were subtyped correctly in accordance with previous examinations performed by classical diagnostic methods. Testing of heterologous pathogens verified the specificity of the assay. This assay is a convenient and practical tool for the study of AIVs, providing important HA and NA data more rapidly than conventional methods.
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Conze, TimUppsala universitet,Molekylära verktyg(Swepub:uu)ticon367
(author)
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Zohari, Siamak
(author)
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LeBlanc, Neil
(author)
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Nilsson, MatsUppsala universitet,Molekylära verktyg(Swepub:uu)matsnils
(author)
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Landegren, UlfUppsala universitet,Molekylära verktyg(Swepub:uu)ulfland
(author)
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Banér, Johan
(author)
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Belák, Sándor
(author)
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Uppsala universitetMolekylära verktyg
(creator_code:org_t)
Related titles
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In:Journal of Clinical Microbiology46:5, s. 1747-17510095-11371098-660X
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