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The role of oxygen ...
The role of oxygen termination of nanocrystalline diamond on immobilisation of BMP-2 and subsequent bone formation
- Article/chapterEnglish2008
Publisher, publication year, extent ...
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Elsevier BV,2008
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printrdacarrier
Numbers
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LIBRIS-ID:oai:DiVA.org:uu-16546
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https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-16546URI
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https://doi.org/10.1016/j.biomaterials.2008.01.036DOI
Supplementary language notes
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Language:English
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Summary in:English
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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Medical implants are increasingly often inserted into bone of frail patients, who are advanced in years. Due to age, severe trauma or pathology-related bone changes, osseous healing at the implant site is frequently limited. We were able to demonstrate that coating of endosseous implants with nanocrystalline diamond (NCD) allows stable functionalization by means of physisorption with BMP-2. Strong physisorption was shown to be directly related to the unique properties of NCD, and BMP-2 in its active form interacted strongly when NCD was oxygen-terminated. The binding of the protein was monitored under physiological conditions by single molecule force spectroscopy, and the respective adsorption energies were further substantiated by force-field-calculations. Implant surfaces refined in such a manner yielded enhanced osseointegration in vivo, when inserted into sheep calvaria. Our results further suggest that this technical advancement can be readily applied in clinical therapies with regard to bone healing, since primary human mesenchymal stromal cells strongly activated the expression of osteogenic markers when being cultivated on NCD physisorbed with physiological amounts of BMP-2.
Subject headings and genre
Added entries (persons, corporate bodies, meetings, titles ...)
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Gassner, Robert
(author)
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Preiner, Johannes
(author)
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Ebner, Andreas
(author)
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Larsson, KarinUppsala universitet,Oorganisk kemi(Swepub:uu)karinl
(author)
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Hächl, Oliver
(author)
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Tuli, Tarkan
(author)
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Rasse, Michael
(author)
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Moser, Doris
(author)
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Laimer, Klaus
(author)
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Nickel, Eike
(author)
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Laschober, Gerhard
(author)
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Brunauer, Regina
(author)
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Klima, Günther
(author)
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Hinterdorfer, Peter
(author)
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Steinmüller-Nethl, Doris
(author)
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Lepperdinger, Günter
(author)
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Uppsala universitetOorganisk kemi
(creator_code:org_t)
Related titles
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In:Biomaterials: Elsevier BV29:16, s. 2433-24420142-96121878-5905
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- By the author/editor
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Kloss, Frank
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Gassner, Robert
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Preiner, Johanne ...
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Ebner, Andreas
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Larsson, Karin
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Hächl, Oliver
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Tuli, Tarkan
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Rasse, Michael
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Moser, Doris
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Laimer, Klaus
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Nickel, Eike
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Laschober, Gerha ...
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Brunauer, Regina
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Klima, Günther
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Hinterdorfer, Pe ...
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Steinmüller-Neth ...
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Lepperdinger, Gü ...
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Chemical Science ...
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and Inorganic Chemis ...
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Biomaterials
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Uppsala University