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Polypeptide Folding-Mediated Tuning of the Optical and Structural Properties of Gold Nanoparticle Assemblies
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- Aili, Daniel (author)
- University of London Imperial College of Science Technology and Medicine
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- Gryko, Piotr (author)
- University of London Imperial College of Science Technology and Medicine
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- Sepulveda, Borja (author)
- Research Centre Nanosci and Nanotechnol CIN2 CSIC
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- (creator_code:org_t)
- 2011-11-08
- 2011
- English.
- In: Nano letters (Print). - : American Chemical Society (ACS). - 1530-6984 .- 1530-6992. ; 11:12, s. 5564-5573
- Journal article (peer-reviewed)
Abstract
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- Responsive hybrid nanomaterials with well-defined properties are of significant interest for the development of biosensors with additional applications in tissue engineering and drug delivery. Here, we present a detailed characterization using UV-vis spectroscopy and small angle X-ray scattering of a hybrid material comprised of polypeptide-decorated gold nanoparticles with highly controllable assembly properties. The assembly is triggered by a folding-dependent bridging of the particles mediated by the heteroassociation of immobilized helix-loop-helix polypeptides and a complementary nonlinear polypeptide present in solution. The polypeptides are de novo designed to associate and fold into a heterotrimeric complex comprised of two disulfide-linked four-helix bundles. The particles form structured assemblies with a highly defined interparticle gap (4.8 +/- 0.4 nm) that correlates to the size of the folded polypeptides. Transitions in particle aggregation dynamics, mass-fractal dimensions and ordering, as a function of particle size and the concentration of the bridging polypeptide, are observed; these have significant effects on the optical properties of the assemblies. The assembly and ordering of the particles are highly complex processes that are affected by a large number of variables including the number of polypeptides bridging the particles and the particle mobility within the aggregates. A fundamental understanding of these processes is of paramount interest for the development of novel hybrid nanomaterials with tunable structural and optical properties and for the optimization of nanoparticle-based colorimetric biodetection strategies.
Keyword
- Gold nanoparticles
- polypeptides
- folding
- helix-loop-helix
- small angle X-ray scattering (SAXS)
- MEDICINE
Publication and Content Type
- ref (subject category)
- art (subject category)
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- Aili, Daniel
- Gryko, Piotr
- Sepulveda, Borja
- Dick, John A. G.
- Kirby, Nigel
- Heenan, Richard
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- Baltzer, Lars
- Liedberg, Bo
- Ryan, Mary P.
- Stevens, Molly M ...
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- Uppsala University
- Linköping University
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