Targeted enrichment of genomic DNA regions for next-generation sequencing

Mertes, Florian (author)

ElSharawy, Abdou (author)

Sauer, Sascha (author)

van Helvoort, Joop M. L. M. (author)

van der Zaag, P. J. (author)

Franke, Andre (author)

Lehrach, Hans (author)

Brookes, Anthony J. (author)

(creator_code:org_t)

2011-11-26
2011
English.
In: Briefings in functional genomics. - : Oxford University Press (OUP). - 2041-2649 .- 2041-2657. ; 10:6, s. 374-386

Related links:: https://academic.oup...; show more...; https://urn.kb.se/re...; https://doi.org/10.1...; show less...

Abstract Subject headings

In this review, we discuss the latest targeted enrichment methods and aspects of their utilization along with second-generation sequencing for complex genome analysis. In doing so, we provide an overview of issues involved in detecting genetic variation, for which targeted enrichment has become a powerful tool. We explain how targeted enrichment for next-generation sequencing has made great progress in terms of methodology, ease of use and applicability, but emphasize the remaining challenges such as the lack of even coverage across targeted regions. Costs are also considered versus the alternative of whole-genome sequencing which is becoming ever more affordable. We conclude that targeted enrichment is likely to be the most economical option for many years to come in a range of settings.

By the author/editor: Mertes, Florian; ElSharawy, Abdou; Sauer, Sascha; van Helvoort, Jo ...; van der Zaag, P. ...; Franke, Andre; show more...; Nilsson, Mats; Lehrach, Hans; Brookes, Anthony ...; show less...

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.