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Amino acid sequence...
Amino acid sequence at the phosphorylated site of rat liver fructose-1,6-diphosphatase and phosphorylation of a corresponding synthetic peptide
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- Humble, Elisabet (author)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi,engström lorentz
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- Dahlqvist-Edberg, Ulla (author)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi,engström lorentz
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- Ek, Pia (author)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi,engström lorentz
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- Netzel, Elvy (author)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi,engström lorentz
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- Ragnarsson, Ulf (author)
- Uppsala universitet,Biokemi,Institutionen för naturvetenskaplig biokemi,ulf ragnarsson
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- Engström, Lorentz (author)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi,engström lorentz
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(creator_code:org_t)
- 1979
- 1979
- English.
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In: Biochemical and Biophysical Research Communications - BBRC. - 0006-291X .- 1090-2104. ; 90:3, s. 1064-1072
- Related links:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
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- Rat liver fructose-1,6-diphosphatase was phosphorylated with (32P)ATP and the catalytic subunit of cyclic AMP-dependent protein kinase from pig muscle. After digestion with pepsin, α-chymotrypsin and subtilisin a peptide with the amino-terminal sequence Ser-Arg-Tyr-(32P)SerP-Leu-Pro-Leu-Pro was isolated. A synthetic unphosphorylated heptapeptide with the same amino acid sequence, ending with leucine, was phosphorylated with an apparent Km of 400 μM, while the apparent Km value for fructose-1,6-diphosphatase was 30 μM (subunit concentration). The Vmax value was 20 times higher for the peptide than for the enzyme.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Annan medicin och hälsovetenskap (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Other Medical and Health Sciences (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Annan medicin och hälsovetenskap -- Övrig annan medicin och hälsovetenskap (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Other Medical and Health Sciences -- Other Medical and Health Sciences not elsewhere specified (hsv//eng)
Publication and Content Type
- ref (subject category)
- art (subject category)
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